目的 研究川楝子Melia toosendan 95%乙醇提取物中柠檬苦素类的化学成分及抗肿瘤活性。方法 采用常压柱色谱、高压柱色谱和凝胶柱色谱等多种分离技术进行分离纯化，并利用现代波谱技术对分离得到的化合物进行结构表征。同时通过CCK-8法评估其抗肿瘤活性。结果 从川楝子醋酸乙酯萃取部分共分离到8个柠檬苦素型三萜化合物，通过波谱技术分别鉴定为15-oxotrichilinin（1）、11,15-dioxotrichilinin（2）、12-O-ethyl nimbolinin B（3）、mufolinoid U（4）、trichilinin（5）、trichilinin D（6）、trichilinin E（7）、trichilinin G（8）。活性评价结果显示各化合物对4种肿瘤细胞均具有不同程度的抑制作用。结论 化合物1为新的化合物，命名为川楝子酮。抗肿瘤活性表明，化合物1～8均有不同程度的抗肿瘤活性，部分化合物显示对HepG2细胞有良好抑制活性。

Objective To investigate the chemical constituents of limonoid-type triterpenes from the 95% ethanol extract of Melia toosendan and their antitumor activity. Methods The compounds were isolated and purified using various chromatographic techniques, including normal-pressure column chromatography, high-pressure column chromatography, and gel column chromatography. The isolated compounds were structurally characterized by modern spectroscopic techniques. Their antitumor activity was evaluated using the CCK-8 assay. Results Eight limonoid-type triterpenoids were isolated from the ethyl acetate extract of M. toosendan. Structural elucidation by spectroscopic techniques identified them as 15-oxotrichilinin (1), 11,15-dioxotrichilinin (2), 12-O-ethyl nimbolinin B (3), mufolinoid U (4), trichilinin (5), trichilinin D (6), trichilinin E (7), and trichilinin G (8). Activity evaluation results showed that all compounds exhibited varying degrees of inhibitory effects on four tumor cell lines. Conclusion Compound 1 is a new compound, named 15-oxotrichilinin. Antitumor activity results indicated that compounds 1—8 all possess antitumor activity to varying extents, with some compounds showing favorable activity against HepG2 cells.

R284.1

四川省高等学校省级高阶课程项目（2025SJGJKC01）；四川省教改项目（JG2024-1131）