目的 研究砂茴香Ferula bungeana全草石油醚提取物的化学成分。方法 运用经典硅胶柱色谱及薄层色谱对砂茴香全草石油醚提取物的化学成分进行研究，并对分离得到化合物的波谱数据进行分析，确定其结构。同时，通过考察分离得到化合物对脂多糖（lipopolysaccharide，LPS）诱导的小鼠RAW264.7巨噬细胞培养液中一氧化氮（NO）释放量的影响，评价其对炎症反应的抑制作用。结果 从砂茴香全草石油醚提取物中分离鉴定了8个苯丙烷类化合物，分别为1-异戊烯酰氧基-1-(3'-羟基-4',5'-亚甲二氧基)苯基-2-当归酰氧基丙烷（1）、肉豆蔻醚（2）、深黄水芹酮（3）、diversivittatin（4）、2-epilaserine（5）、1-羟基-1-(3'-甲氧基-4',5'-亚甲二氧基)苯基丙烷（6）、1-羟基-1-(3',4'-亚甲二氧基-2',5'-二甲氧基)苯基丙烷（7）、1-羰基-1-(3'-羟基-4',5'-亚甲二氧基)苯基丙烷（8）。活性筛选结果显示，在10 μmol/L浓度下，化合物1、4和5具有良好的NO抑制活性，其抑制率分别为78.5%、85.9%和79.0%。结论 化合物1为新化合物，命名为砂茴香二酯；化合物2和3为首次从砂茴香中分离得到，而化合物4～8均为首次从阿魏属植物中分离得到。化合物1、4和5具有良好的抗炎活性。

Objective To clarify the chemical constituents of the petroleum ether extract from the whole plant of Ferula bungeana. Methods The chemical constituents of petroleum ether extract from the whole plant of F. bungeana were studied using classical silica gel column chromatography and thin-layer chromatography. The spectral data of the isolated compounds were analyzed to determine their structures. Meanwhile, by examining the effect of the isolated compounds on nitric oxide (NO) release in the culture medium of RAW264.7 macrophages induced by lipopolysaccharide (LPS), their inhibitory effect on inflammatory responses was evaluated. Results From the petroleum ether extract of F. bungeana whole plant, eight compounds were isolated and identified as 1-isoprenyloxy-1-(3'-hydroxy-4',5'-methylenedioxy)phenyl-2-angeloyloxy-phenylpropane (1), myristicin (2), crocatone (3), diversivittatin (4), 2-epilaserine (5), 1-hydroxy-1-(3'-methoxy-4',5'-methylenedioxy)-phenylpropane (6), 1-hydroxy-1-(3',4'-methylenedioxy-2',5'-dimethoxy)-phenylpropane (7), 1-carbonyl-1-(3'-hydroxy-4',5'-methylenedioxy)-phenylpropane (8). The activity screening results showed that compounds 1, 4, and 5 exhibited good NO inhibitory activity at a concentration of 10 μmol/L, with inhibition rates of 78.5%, 85.9%, and 79.0%, respectively. Conclusions Compound 1 is a new compound, named ferubundiester; Compounds 2 and 5 were isolated from F. bungeana for the first time, and compounds 4—8 were obtained from plants of the Ferula genus for the first time. Compounds 1, 4, and 5 exhibit similar potent anti-inflammatory activity.

R284.1

内蒙古自治区自然科学基金项目（2025QN08004）；内蒙古自治区教育厅一流学科专项项目（YLXKZX-NMD-004）；蒙药研发国家地方联合工程研究中心开放基金项目（MDK2024010）