目的 研究适宜当归Angelicas sinensis生长和活性物质积累的光质处理，为调控当归生长和代谢产物积累提供参考。方法 对当归盆栽植株进行3种光质处理（红光、蓝光和红外光），以白光为对照（CK），测定植株生长特性、主要活性物质含量及关键基因表达水平。结果 不同光质对当归生长特性、主要活性物质积累和关键基因表达存在较大差异；与CK相比，蓝光显著促进株高、根长、叶绿素含量、气孔开度，红光和红外光在一定程度上可促进某一生长参数（如株高和地上干质量），但蓝光、红光和红外光对生物量积累不显著；红光显著促进可溶性糖积累，蓝光显著促进总黄酮积累，而红外光显著促进阿魏酸、藁本内酯、总酚类积累和体外抗氧化能力；3种光质处理均可促进主要活性物质（阿魏酸、黄酮类、挥发油及多糖类）生物合成多数相关基因表达水平，比如，参与阿魏酸生物合成的咖啡酸3-O-甲基转移酶（caffeic acid 3-O-methyltransferase，COMT）基因在蓝光处理最为显著，参与黄酮类生物合成的查耳异构酶2（chalcone isomerase 2，CHI2）基因在红外光处理最为显著，参与挥发油类生物合成的1-脱氧-D-木酮糖-5-磷酸合成酶（1-deoxy-D-xylulose 5-phosphate synthase，DXS）基因在红光处理最为显著。结论 单色光质（蓝光、红光和红外光）对当归生长和主要活性物质积累存在特异性效应，因此，对复合光调控当归生长和代谢产物积累效应需进一步研究。

Objective To find out the optimal light quality for plant growth and bioactive metabolites accumulation of Angelica sinensis, and provide references for regulating the growth and metabolites product accumulation of A. sinensis. Methods The A. sinensis plants grown in pots were exposed to three different light qualities (red, blue, and infrared light) with white light as the control (CK), the growth characteristics of plants, contents of main bioactive metabolites, and expression level of key genes were determined. Results There were significant differences in the growth characteristics, main bioactive metabolites accumulation, and key genes expression of A. sinensis under different light qualities. Compared with the CK, blue light significantly promoted plant height, root length, chlorophyll content, and stomatal aperture; red light and infrared light could promote some growth parameters (e.g., plant height and aerial parts dry weight) to some extent; however, the blue, red, or infrared light had no significant effect on biomass accumulation. Red light significantly promoted soluble sugar accumulation, blue light significantly promoted total flavonoids accumulation, while infrared light significantly promoted accumulation of ferulic acid, ligustilide and total phenolics as well as in vitro antioxidant capacity. The three light qualities could promote the expression levels of almost genes related to the biosynthesis of main bioactive metabolites (i.e., ferulic acid, flavonoids, volatile oils, and polysaccharides); for example, the caffeic acid 3-O-methyltransferase (COMT) gene involved in ferulic acid biosynthesis showed the highest upregulation under blue light, the chalcone isomerase 2 (CHI2) gene involved in flavonoid biosynthesis was significantly upregulated under infrared light; and the 1-deoxy-D-xylulose 5-phosphate synthase (DXS) gene involved in volatile oil biosynthesis was significantly upregulated under red light. Conclusion Monochromatic light qualities (blue, red, and infrared light) presented specific effects on the growth and main bioactive metabolites accumulation in A. sinensis, thus, the regulatory effect of combined lights on the growth and metabolites accumulation requires further study.

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国家自然科学基金资助项目(32360100);国家自然科学基金资助项目(32160083); 国家现代农业产业技术体系(CARS-21); 云南省科技计划项目(202304B1090009); 甘肃省科技重大专项(23ZDNK002); 甘肃省重点研发计划(22YF7NA111)