目的 研究鬼箭羽（卫矛Euonymus alatus带栓翅的枝条）的化学成分。方法 采用正反相硅胶、Sephadex LH-20和高效液相等色谱等方法进行分离纯化，并运用NMR、MS、X-Ray等技术鉴定所得化合物的结构，采用CCK-8法评价化合物改善足细胞损伤作用。结果 从鬼箭羽95%乙醇提取物的二氯甲烷部位中分离纯化得到6个化合物，分别鉴定为3β-hydroxy-24-norhopa-4(23)（1）、乙酰乌索酸（2）、齐墩果酮酸（3）、foliasalaci A4（4）、白桦脂酮酸（5）、乌苏酸内酯（6）。且化合物1对糖基化终末产物（advanced glycation end products，AGEs）诱导的MPC5细胞损伤有明显的改善作用。结论 化合物1为新化合物，命名为2,4-降白桦脂醇；其余化合物则均为首次从该植物及该属植物中分离得到。化合物1显示出抗足细胞损伤作用，且具有浓度相关性。

Objective To investigate the chemical constituents of Euonymus alatus. Methods Separation and purification were performed using normal- and reversed-phase silica gel, Sephadex LH-20, and high-performance liquid chromatography. The structures of the obtained compounds were identified using NMR, MS, and X-Ray techniques. The protective effect of the compound against podocyte injury was evaluated using the CCK-8 assay. Results Six compounds were isolated and purified from the dichloromethane fraction of the 95% ethanol extract of E. alatus, namely 3β-hydroxy-24-norhopa-4(23) (1), acetyl ursolic acid (2), betulinic acid (3), foliasalaci A4 (4), betulonicacid (5), and ursolic acid lactone (6). Moreover, compound 1 has a significant protective effect against MPC5 cell damage induced by advanced glycation end-products (AGEs). Conclusion Compound 1 is a new compound, named 2,4-dehydrowhite birch resinol. All the other compounds were isolated from this plant and plants of the same genus. Compound 1 exhibits anti-podocyte injury activity in a concentration-dependent manner.

R284.1

国家重点研发计划资助项目(2020YFE0201800); 国家自然科学基金面上项目(82474495); 河南省高校科技创新人才(24HASTIT072)