目的 研究羊角棉Alstonia mairei枝叶的化学成分及抗真菌活性。方法 采用MCI、硅胶、羟丙基葡聚糖凝胶及制备高效液相等多种色谱方法进行分离纯化，利用质谱、核磁共振和ECD等谱学方法鉴定化合物结构。采用微量肉汤稀释法测试了部分单体化合物对白色念珠菌（Candida albicans CMCC98001）和黑曲霉菌（Aspergillus niger R330）的抗菌活性。结果 从羊角棉枝叶中分离并鉴定了8个化合物，包括1个新化合物ent-12-羟基-16-羟甲基阿替烷-3-酮（1）以及7个已知化合物，分别为17-乙酰蛇根精（2）、1,2,3,4-四氢-1-氧-β-咔啉（3）、维诺任碱N₄-氧化物（4）、维诺任碱（5）、育亨宾（6）、华北白前醇（7）、ent-16β,17-二羟基阿替烷-3-酮（8）。化合物1和8对黑曲霉菌的最低抑菌浓度（minimum inhibitory concentration，MIC）值分别为12.5、6.25 μg/mL。结论 化合物1为新的二萜类化合物，命名为替烷醇酮A，化合物3、7和8是首次从鸡骨常山属植物中分离获得。化合物1和8对黑曲霉菌有一定的抑制作用。

Objective To investigate the chemical constituents from the twigs and leaves of Alstonia mairei and to evaluate their antifungal activity. Methods The separation and purification were conducted by a variety of chromatographic separation techniques, including MCI, silica gel, Sephadex LH-20 gel column chromatography, and preparative high-performance liquid chromatography. The chemical structures of the isolated compounds were elucidated by spectroscopic methods, including mass spectrometry, nuclear magnetic resonance, and electronic circular dichroism (ECD). The antifungal activity of the selected compounds was evaluated against Candida albicans CMCC98001 and Aspergillus niger R330 using the microbroth dilution method. Results A total of eight compounds were isolated from the twigs and leaves of A. mairei, including one new compound, ent-12-hydroxy-16-methylol-atisane-3-one (1), and seven known compounds, 17-acetylsarpagine (2), 1,2,3,4-tetrahydro-1-oxo-β-carboline (3), vinorine N₄-oxide (4), vinorine (5), yohimbine (6), hancockinol (7), ent-16β, 17-dihydroxyatlsan-3-one (8). The MICs of compound 1 and 8 against A. niger R330 were 12.5 and 6.25 μg/mL, respectively. Conclusion Compound 1 is a new diterpenoid named alstomanoid A, and compounds 3, 7 and 8 are isolated from the genus of A. mairei for the first time. Compounds 1 and 8 show antifungal effect against A. niger R330.

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