目的 建立不同产地加工品种浙贝母Fritillaria thunbergii水溶性成分的HPLC指纹图谱，对不同产地的浙贝片和灰贝的质量进行评价。方法 采用Inertsil ODS-SP-C₁₈色谱柱（250 mm×4.6 mm，5.0 μm），甲醇（A）-水（B）为流动相进行梯度洗脱；检测波长260 nm，柱温25 ℃，体积流量1.0 mL/min，进样量15 μL。建立HPLC指纹图谱并分析相似度，结合对照品对比指认化学成分并进行定量测定，采用聚类分析（hierarchical cluster analysis，HCA）、主成分分析（principal components analysis，PCA）和正交偏最小二乘法判别分析（orthogonal partial least squares discrimination analysis，OPLS-DA）评价浙贝母不同产地加工品种浙贝片和灰贝质量。结果 浙贝片和灰贝的指纹图谱共匹配了15个共有峰，指认了其中10个成分，分别为峰3（尿嘧啶）、峰5（次黄嘌呤）、峰6（鸟嘌呤）、峰8（胸腺嘧啶）、峰9（肌苷）、峰10（鸟苷）、峰11（腺嘌呤）、峰12（胸苷）、峰14（腺苷）、峰15（2-脱氧腺苷），进一步采用HCA、PCA、OPLS-DA对15个共有峰峰面积进行分析，结果均能将浙贝片与灰贝很好的区分，以VIP＞1为指标共出8个贡献率较大的差异性成分，分别为峰3、7、10、5、12、14、6、8。结论 建立的浙贝片和灰贝水溶性成分的指纹图谱和多成分含量测定方法操作简便，且重复性好，可用于浙贝母不同产地加工方式的质量评价及质量控制。

Objective To establish the HPLC fingerprint of water-soluble components for different habitat‑processed products of Fritillaria thunbergii, and evaluate the quality of F. thunbergii piece and Huibei from different origins. Methods Inertsil ODS-SP-C₁₈column (250 mm × 4.6 mm, 5.0 μm) was used with methanol (A)-water (B) as mobile phase for gradient elution. The detection wavelength was 260 nm, the column temperature was 25 °C, the volume flow rate was 1.0 mL/min, and the injection volume was 15 μL. The HPLC fingerprint was established and the similarity was analyzed. The chemical constituents were identified and quantitatively determined by comparing with the reference substances. Hierarchical cluster analysis (HCA), principal components analysis (PCA) and orthogonal partial least squares discrimination analysis (OPLS-DA) were used to evaluate the quality of F. thunbergii from different producing areas. Results A total of 15 common peaks were matched in the fingerprints of F. thunbergii piece and Huibei, and ten components were identified including peak 3-uracil, peak 5-hypoxanthine, peak 6-guanine, peak 8-thymine, peak 9-inosine, peak 10-guanosine, peak 11-adenine, peak 12-thymidine, peak 14-adenosine and peak 15-2-deoxyadenosine. The areas of 15 common peaks were further analyzed by HCA, PCA and OPLS-DA. The results can distinguish F. thunbergii piece and Huibei well. With VIP > 1 as the index, a total of eight differential components with large contribution rate were obtained, which were peak 3,7,10,5,12,14,6 and 8. Conclusion The established fingerprint and multi-component content determination methods of water-soluble components of F. thunbergii piece and Huibei are simple and reproducible, and can be used for the quality evaluation and quality control of different processing methods of F. thunbergii from different producing areas.

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浙江省自然科学基金项目（LTGN23H280001）；杭州市自然科学基金项目（2024SZRYBC200002）；杭州市农业与社会发展科研重点项目（202204A06）；宁波市“科技创新2025重大专项”（2020Z089）