目的 研究骆驼刺Alhagi sparsifolia醋酸乙酯部位的化学成分。方法 采用硅胶、凝胶、ODS等柱色谱结合半制备液相色谱技术进行分离纯化，通过现代波谱学技术对化合物进行结构鉴定。同时采用MTT法测试各化合物抗人宫颈癌HeLa细胞的活性。结果 从骆驼刺醋酸乙酯提取物中共分离得到15个化合物，分别鉴定为1-[1-[4-(4-羟基苯乙氧基)苯基]乙基]-1H-吡咯-2-基]乙酮（1）、5,7-二羟基-6-甲氧基黄酮（2）、callyspongidipeptide A（3）、环D-脯氨酸-D-缬氨酸（4）、7-O-乙基愈创木基甘油（5）、7-O-乙基愈创木基甘油（6）、isololiolide（7）、N-苯乙基乙酰胺（8）、黄芩新素Ⅱ（9）、4-羟基苯乙醇（10）、N-苯乙基甲酰胺（11）、3'-甲氧基大豆苷元（12）、环(丙氨酸-脯氨酸)（13）、N-(对羟基苯乙基)甲酰胺（14）、N-乙酰基酪胺（15）。结论 化合物1为新化合物，命名为骆驼刺吡咯碱A。化合物1、5和6具有抗宫颈癌活性，对HeLa细胞半数抑制浓度（median inhibition concentration，IC₅₀）值分别为31.4、26.6、37.3 μmol/L。

Objective To study the chemical constituents from the ethyl acetate extract of Alhagi sparsifolia Shap. Methods The compounds were isolated and purified by silica gel, Sephadex LH-20, ODS column chromatography, and semi‑preparative HPLC, and their structures were identified by modern spectroscopic techniques. The anti‑cervical cancer activities of the compounds against human HeLa cells were evaluated by the MTT assay. Results Fifteen compounds were isolated from the ethyl acetate extract of A. sparsifolia and identified as 1-{1-[4-(4-hydroxyphenethoxy)phenethyl]-1H-pyrrol-2-l}ethan-1-one(1), 5,7-dihydroxy-6-methoxyflavone (2), callyspongidipeptide A (3), cyclo-(D-proline-D-valine) (4), 7-O-ethylguaiacyl glycerol (5), 7-O-ethylguaiacyl glycerol (6), isololiolide (7), N-phenethylacetamide (8), skullcapflavone II (9), 4-hydroxyphenethyl alcohol (10), N-phenethylformamide (11), 3′-methoxydaidzein (12), cyclo(alanine-proline) (13), N-(p-hydroxyphenethyl)formamide (14), and N-acetyltyramine (15). Conclusions Compound 1 is a new compound and named as alhagipyrrolizidine A. Compounds 1, 5 and 6 showed significant activity against human HeLa cells, with IC₅₀ values of 31.4, 26.6 and 37.3 μmol/L, respectively.

R284.1

新疆维吾尔自治区重点研发计划项目（2022B03018-4）