目的 对比不同来源及不同工艺所得的三七Panax notoginseng茎叶提取物（DRNJE）中皂苷、黄酮、多糖含量的差别及其调血脂作用。方法 以溶剂梯度萃取法石油醚脱脂、醋酸乙酯提取黄酮、水饱和正丁醇提取皂苷，多糖水溶液按氯仿-正丁醇-水为25∶4∶1除去蛋白质。采用紫外分光光度计法测定DRNJE中皂苷、黄酮、多糖含量并采用HPLC法测定人参皂苷Rb₃、人参皂苷Rb₁、人参皂苷Rc的含量。采用胰脂肪酶及油酸诱导的人肝癌HepG2细胞对DRNJE进行调血脂作用的研究。结果 大孔树脂/离子交换脱色处理后Y0729、J0801、J1020、Y1114、J1031、R1001的总皂苷含量高、单体皂苷含量高、总体功效成分含量高。对于脂肪酶抑制活性由高到低为Y0729＞J1031＞J1020＞R1001＞Y1114＞J0801＞Q0901＞Q1001＞W1107＞W1101。根据油红O染色结果可知，DRNJE均具有调血脂作用，其中Y0729、J1020、J1031、R1001效果较好。结论 经大孔树脂/离子交换脱色处理后的DRNJE其皂苷、黄酮、功效成分含量高，其中皂苷含量高的DRNJE对脂肪酶和油酸诱导的HepG2细胞调血脂作用效果显著。

Objective To compare the contents of saponins, flavonoids and polysaccharides in the extracts of stems and leaves of Panax notoginseng from different sources and different processes (DRNJE) and to study their lipid-regulating effects. Methods The solvent gradient extraction method was used to remove protein by degreasing with petroleum ether, extracting flavonoids with ethyl acetate, extracting saponins with water-saturated n-butanol and adjusting the ratio of chloroform to n-butanol to 25∶4∶1. The contents of saponins, flavonoids and polysaccharides in the stem and leaf extracts of P. notoginseng were determined by ultraviolet spectrophotometer, and the contents of monomer saponins Rb₃, Rb₁ and Rc were simultaneously detected by HPLC. HepG2 cells induced by pancreatic lipase and oleic acid were used to study the lipid-regulating effect of DRNJE. Results After decolorization by macroporous resin/ion exchange, the contents of total saponins, monomer saponins and overall functional components of P. notoginseng stem and leaf extracts Y0729, J0801, J1020, Y1114, J1031 and R1001 were high. The inhibitory activity of lipase from high to low is: Y0729 > J1031 > J1020 > R1001 > Y1114 > J0801 > Q0901 > Q1001 > W1107 > W1101. According to the results of oil red O staining, DRNJE has lipid-regulating effect, among which Y0729, J1020, J1031 and R1001 have better effects. Conclusion After decolorization by macroporous resin/ion exchange, the extract of P. notoginseng stems and leaves has high content of saponins, flavonoids and polysaccharides, among which the extract of P. notoginseng stems and leaves with high saponin content has a significant effect on lipid regulation of HepG2 cells induced by lipase and oleic acid.

R286.2

延边大学创新团队项目（012023051）