目的 光因子是叶用药用植物生长发育及次生代谢形成的重要生态因子之一，对柔毛淫羊藿Epimedium pubescens MYB转录因子家族（EpMYB）进行全基因组鉴定及表达特征分析，深入研究EpMYB响应光因子下的生物学功能。方法 基于已发布的柔毛淫羊藿全基因组，利用生物信息学方法对EpMYB基因家族成员进行鉴定并对其理化性质、染色体分布、系统进化、基因结构、顺式作用元件进行分析，利用实时荧光定量PCR（RT-qPCR）分析其在不同光质下柔毛淫羊藿叶片表达特征。结果 鉴定出87个EpMYB基因（EpMYB1～EpMYB87），细分为14个亚家族，编码的氨基酸长度在150～561 aa，蛋白质相对分子质量为17 857.76～61 369.56，等电点介于4.62～10.75。基因结构分析发现所有EpMYB基因均含有相似保守结构域，顺式元件预测结果表示光响应元件、茉莉酸甲酯、生长素元件在EpMYB基因家族启动子区域分布广泛。基因组内共线性分析表明，全基因组复制和片段性复制在EpMYB基因家族进化中发挥了重要作用，复制过后经过了强烈的纯化选择。对24个潜在光响应的EpMYB基因进行RT-qPCR表达特征分析，结果表明，黄、蓝光处理下对显著上调表达的EpMYB基因的数量远高于红光，蓝光显著上调EpMYB25等14个EpMYB基因的表达，黄光显著上调EpMYB16等7个EpMYB基因的表达，红光处理下仅EpMYB54、EpMYB57基因显著上调。结论 从全基因组水平对柔毛淫羊藿EpMYB基因家族进行鉴定和生物信息学分析，EpMYB基因对蓝光调控有更积极的响应，为进一步阐明柔毛淫羊藿EpMYB基因的功能奠定基础。

Objective Light is one of the important ecological factors for the growth, development, and secondary metabolism of leaf-using medicinal plants. To conduct genome-wide identification and expression characterization analysis of the MYB transcription factor family (EpMYB) in Epimedium pubescens, and to deeply explore the biological functions of EpMYB in response to light factors. Methods Based on the published genome of E. pubescens, bioinformatics methods were used to identify members of the EpMYB gene family, and analyze their physicochemical properties, chromosomal distribution, phylogenetic evolution, gene structure, and cis-acting elements. Real-time quantitative PCR (RT-qPCR) was applied to analyze the expression characteristics of EpMYB in E. pubescens leaves under different light qualities. Results A total of 87 EpMYB genes (EpMYB1 to EpMYB87) were identified, which were subdivided into 14 subfamilies. The encoded amino acids ranged from 150 to 561 aa, with relative molecular weights of proteins from 17 857.76 to 61 369.56 and isoelectric points between 4.62 and 10.75. Gene structure analysis showed that all EpMYB genes contained similar conserved domains. Cis-element prediction indicated that light-responsive elements, methyl jasmonate, and auxin elements were widely distributed in the promoter regions of the EpMYB gene family. Genome-wide collinearity analysis revealed that whole-genome duplication and segmental duplication played crucial roles in the evolution of the EpMYB gene family, and strong purifying selection occurred after duplication. RT-qPCR analysis of the expression characteristics of 24 potential light-responsive EpMYB genes showed that the number of EpMYB genes significantly upregulated under yellow and blue light was much higher than that under red light. Blue light significantly upregulated the expression of 14 EpMYB genes such as EpMYB25, yellow light significantly upregulated seven EpMYB genes such as EpMYB16, and only EpMYB54 and EpMYB57 were significantly upregulated under red light. Conclusion Genome-wide identification and bioinformatics analysis of the EpMYB gene family in E. pubescens revealed that EpMYB genes respond more actively to blue light regulation, laying a foundation for further clarification of the functions of EpMYB genes in E. pubescens.

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河南省重点研发专项（251111310500）；河南省重点研发专项（31111312700）；河南省重点研发专项（241111310200）；国家自然科学基金资助项目（82104329）；国家自然科学基金资助项目（32401226）；中国博士后科学基金特别资助项目（2014T170252）；河南省国际科技合作项日（242102521056）；河南中医药大学科研苗圃工程资助项目（MP2024-56）