目的 从丹参Salvia miltiorrhiza基因组中克隆SmABCG34基因，对其进行生物信息学和表达特异性分析。方法 基于丹参基因组筛选克隆SmABCG34基因，利用生物信息学在线网站对该基因及其编码蛋白进行理化性质、保守结构域等分析。利用实时荧光定量PCR（qRT-PCR）检测SmABCG34基因的组织表达特性及对脱落酸（abscisic acid，ABA）、反式玉米素（trans-Zeatin，tZ）、聚乙二醇-6000（polyethylene glycol-6000，PEG-6000）胁迫的响应情况。结果 SmABCG34基因CDS大小为4 347 bp，编码1 448个氨基酸；该蛋白包含典型的结合域（nucleotide-binding domain，NBD）-跨膜结构域（transmembrane domain，TMD）-NBD-TMD结构，属于ABCG亚家族全分子转运蛋白；亚细胞定位预测显示该蛋白定位于细胞膜。系统进化分析表明，SmABCG34与西班牙鼠尾草Salvia hispanica亲缘关系较近，蛋白同源性高达91.23%。表达模式分析显示，SmABCG34基因在丹参根、茎、叶、花中均有表达，在花中表达量最高，叶中次之。ABA、tZ、PEG-6000处理后，相比于0 h，SmABCG34基因在12 h时表达均显著上调。结论 SmABCG34基因属于ABCG亚家族成员，对激素和胁迫处理具有明显的响应，为后续探究SmABCG34基因的功能提供了科学依据。

Objective To clone the SmABCG34 gene from the Salvia miltiorrhiza genome and analyze its bioinformatics and expression characteristics. Methods The SmABCG34 gene was cloned from the S. miltiorrhiza genome, and the Conservative Domain and physicochemical properties of the gene and its encoded protein were analyzed by Bioinformatics Online Website. Quantitative real-time PCR (qRT-PCR) was used to detect the tissue expression specificity of SmABCG34 gene and its response to stress induced by abscisic acid (ABA), trans-zeatin (tZ), and polyethylene glycol-6000 (PEG-6000). Results The CDS of the SmABCG34 gene is 4 347 bp in size and encodes 1 448 amino acids; This protein contains a typical NBD-TMD-NBD-TMD structure and belongs to the full-molecule transporter of the ABCG subfamily; subcellular localization shows that this protein was localizted to the cell membrane. Phylogenetic analysis showed that SmABCG34 was closely related to S. hispanica, with a protein homology of up to 91.23 %. The expression pattern analysis revealed that the SmABCG34 gene was expressed in the roots, stems, leaves and flowers of S. miltiorrhiza with the highest expression in the flowers, followed by the leaves. After treatment with ABA, tZ, and PEG-6000, compared to 0 h, the expression of the SmABCG34 gene was significantly upregulated at 12 h. Conclusion The SmABCG34 gene belongs to the ABCG subfamily and shows a significant response to hormone and stress treatments, providing a scientific basis for further exploration of the function of the SmABCG34 gene.

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国家自然科学基金资助（82404791）；山东省自然科学基金项目（ZR2021QH202）；山东省自然科学基金项目（ZR2023MH338）；国家现代农业产业技术体系资助项目（CARS-21）；菏泽市黄河流域生态保护和高质量发展科技创新突破计划项目（KJTP202308）