目的 研究杨树桑黄Sanghuangporus vaninii的化学成分及其对肿瘤坏死因子（tumor necrosis factor-α，TNF-α）诱导人类风湿性关节炎成纤维细胞MH7A的活性。方法 运用多种分离技术，包括硅胶柱色谱、ODS柱色谱及半制备高效液相色谱等，进行分离纯化；采用UV、IR、HRESIMS、NMR、碳谱计算和ECD等波谱技术鉴定化合物结构；采用CCK-8法评价倍半萜化合物抑制肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）诱导的MH7A细胞增殖的活性，以及ELISA法检测炎症因子白细胞介素-6（interleukin-6，IL-6）和白细胞介素-1β（interleukin-1β，IL-1β）水平。结果 从杨树桑黄95%乙醇提取物中分离得到9个化合物，分别鉴定为 (4S,5S,6R)-2-甲基-5,6-二羟基-[R-6-(4-羟甲基)-3-环己烯]-呋喃- 2(5H)-酮（1）、phellilane D（2）、phellilane C（3）、(＋)-γ-ionylideneacetic acid（4）、elgonene A（5）、3E-4-(3,4-二羟苯基)-3-丁烯-2-酮（6）、3,4-二羟基苯甲酸乙酯（7）、E-4-对羟基苯基-3-丁烯-2-酮（8）和原儿茶醛（9）。化合物1、3和4在20 μmol/L浓度内均能显著抑制TNF-α诱导的MH7A细胞增殖，在20 μmol/L时其抑制率分别为（38.9±1.32）%、（46.21±0.86）%和（36.59±1.51）%；化合物1、3和4均能显著降低TNF-α诱导后的MH7A细胞IL-6和IL-1β的生成量。结论 化合物1～5为没药烷型倍半萜化合物，6～9为苯酚类化合物，其中化合物1为新的倍半萜化合物，命名为杨树桑黄萜素Q；化合物7为首次从杨树桑黄中分离得到。化合物1、3和4均能显著抑制TNF-α诱导的MH7A细胞增殖并具有抗炎活性。

Objective To investigate the chemical constituents from Sanghuangporus vaninii and their activity against tumor necrosis factor-α (TNF-α)-induced human rheumatoid arthritis fibroblast synovial cells (MH7A). Methods Isolation and purification were carried out by using various separation techniques such as silica gel, ODS column chromatography, and semi-preparative high-performance liquid chromatography. The structures of isolated compounds were elucidated through comprehensive spectroscopic analyses, such as UV, IR, HRESIMS, NMR, carbon spectrum calculation and ECD. Meanwhile, the activity of sesquiterpene compounds in inhibiting the proliferation of MH7A cells induced by TNF-α was evaluated by the CCK-8 method and the levels of inflammatory cytokines IL-6 and IL-1β were detected by ELISA. Results A total of nine compounds were isolated from 95% ethanol extract of S. vaninii and were identified as (4S,5S,6R)-2-methyl-5,6-dihydroxy-[R-6-(4-hydroxymethyl)-3-cyclohexene]-furan-2(5H)-one (1 ), phellilane D (2 ), phellilane C (3 ), (＋)-γ-ionylideneacetic acid (4 ), elgonene A (5 ), (3E)-4-(3,4-dihydroxyphenyl)-but-3-en-2-one (6 ), 3,4-dihydroxybenzoate (7 ), (E)-4-(4-hydroxyphenyl)but-3-en-2-one (8 ), protocatechualdehyde (9 ), respectively. The bioactivity results showed that compounds1 ,3 and4 significantly inhibited the proliferation of TNF-α-induced MH7A cells within 20 μmol/L, and the inhibition rates were (38.9 ± 1.32)%、(46.21 ± 0.86)% and (36.59 ± 1.51)% at 20 μmol/L, respectively. Furthermore, the ELISA results showed they effectively reduced the production of IL-6 and IL-1β on TNF-α-induced MH7A cells. Conclusion Compounds1 —5 were identified as bisabolane type sesquiterpenoids and6 —9 were identified as phenols. Compound1 was a new sesquiterpenoid and named as phellilane Q, Compound7 was first isolated from S. vaninii. Compounds1 ,3 and4 significantly suppressed the proliferation of TNF-α-induced MH7A cells and demonstrated anti-inflammatory effects.

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国家自然科学基金资助项目（U2202213）；国家自然科学基金资助项目（32470428）