目的 系统解析乌天麻Gastrodia elata f.glauca TIFY基因家族成员，并探究其在乌天麻-蜜环菌Armillaria gallica互作过程中的潜在分子机制。方法 基于乌天麻全基因组数据，结合生物信息学方法系统分析其系统进化、基因结构及染色体分布，通过qRT-PCR检测基因表达特征，利用质谱检测茉莉酸（jasmonic acid，JA）含量，分析基因表达与JA含量的相关性。结果 共鉴定到30个乌天麻TIFY家族成员，分属TIFY、JAZ、PPD与ZML 4个亚家族，具有高度保守的蛋白基序和相似的外显子/内含子结构，不均匀分布于7条染色体上，与石斛Dendrobium nobile的保守同源基因对多于拟南芥Arabidopsis thaliana，存在特异性扩张。顺式作用元件分析发现，乌天麻TIFY家族成员启动子区富集大量激素响应、胁迫响应及生长发育相关元件。转录组分析表明，蜜环菌侵染诱导乌天麻19个TIFY家族成员差异表达，母麻中GeJAZ4-3、GeJAZ8、GeJAZ1-1显著上调表达，且多个成员表达量与JA含量呈显著正相关，尤以GeJAZ4-3表达量变化最为显著。结论 乌天麻TIFY基因家族呈现特异性扩张，GeJAZ4-3作为关键调控因子，通过介导JA信号通路调控乌天麻-蜜环菌互作平衡，为天麻共生机制解析及抗病育种奠定重要基础。

ObjectiveTo systematically analyze the TIFY gene family members in Gastrodia elata f. glauca and explore their potential molecular mechanisms in the interaction between G. elata. f. glauca and Armillaria gallica. Methods Based on the whole-genome data of G. elata f. glauca, bioinformatics methods were used to analyze the system evolution, gene structures, and chromosome distribution. The gene expression characteristicswere detected by qRT-PCR, and the jasmonic acid (JA) content was detected by mass spectrometry. The correlation between gene expression and JA content was further analyzed. Results A total of 30 TIFY family members were identified in G. elata f. glauca, belonging to four subfamilies: TIFY, JAZ, PPD, and ZML. They have highly conserved protein motifs and similar exon/intron structures, and are unevenly distributed on seven chromosomes. The number of conserved homologous genes in G. elata f. glauca is more than that in Arabidopsis thaliana, indicating a specific expansion of the TIFY family. The cis-acting element analysis revealed that the promoter regions of the TIFY family members in G. elata f. glauca are enriched with a large number of hormone response, stress response, and growth and development-related elements. Transcriptome analysis showed that A. gallica infection induced differential expression of 19 TIFY family members in G. elata f. glauca. In the mother tubers, GeJAZ4-3, GeJAZ8, and GeJAZ1-1 were significantly upregulated, and the expression levels of multiple members were significantly positively correlated with JA content, especially the expression level of GeJAZ4-3. Conclusion The TIFY gene family in G. elata f. glauca shows specific expansion. GeJAZ4-3, as a key regulatory factor, mediates the JA signaling pathway to regulate the balance of the interaction between G. elata f. glauca and A. gallica, laying an important foundation for the analysis of the symbiotic mechanism of G. elata and facilitating disease-resistant breeding.

R286.12

国家重点研发计划（2023YFC3503803）;中央本级重大增减支项目（2060302）