目的 研究隔山牛皮消Cynanchum wilfordii的化学成分及其抗炎活性。方法 采用硅胶、ODS和Sephadex LH-20等多种色谱分离技术开展化合物的分离纯化，运用HR-ESI-MS、ESI-MS、¹H-NMR、¹³C-NMR、HSQC、HMBC多种波谱技术鉴定化合物结构；采用Griess法研究化合物的抗炎活性。结果 从隔山牛皮消块根中分离得到2个化合物，分别鉴定为(7'R,8'S)-dehydrodiconiferyl alcohol 9'-O-β-D-xylopyranoside（1）和3β-[(O-β-D-glucopyranosyloxy]-8β,14β-trihydroxycarda-5,20(22)-dienolide（2）。化合物1和2对NO生成的抑制率分别为55.16%和15.34%，化合物1的半数抑制浓度（median inhibition concentration，IC₅₀）为41.37 μmol/L。结论 化合物1与2均为新化合物，分别属于木脂素和强心苷类，分别命名为隔山牛皮消素A和隔山牛皮消强心苷A。体外抗炎实验表明，化合物1具有一定的抗炎活性。

Objective To investigate the chemical constituents and their anti-inflammatory activity of Cynanchum wilfordii. Methods Various chromatographic techniques including silica gel, ODS, and Sephadex LH-20 column chromatography were employed for the isolation and purification of compounds. Their structures were elucidated through comprehensive spectroscopic analysis including HR-ESI-MS, ESI-MS, ¹H-NMR, ¹³C-NMR, HSQC, and HMBC. The anti-inflammatory activity of the compounds was evaluated using the Griess method. Results Two compounds were isolated and identified as (7'R,8'S)-dehydrodiconiferyl alcohol 9'-O-β-D-xylopyranoside (1) and 3β-[(O-β-D-glucopyranosyl)-oxy]-8β,14β-trihydroxycarda-5,20-(22)-dienolide (2). Compounds 1 and 2 inhibited NO production by 55.16% and 15.34%, respectively, with a median inhibition concentration (IC₅₀) of 41.37 μmol/L for compound 1. Conclusion Compounds 1 and 2 are both new compounds, belonging to lignan and cardenolide types, respectively. They have been assigned the names cynawilforin A (1) and wilforcardenoside A (2). In vitro anti-inflammatory assays indicated that compound 1 exhibits certain anti-inflammatory activity.

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云南省高校服务重点产业科技项目（FWCY-QYCT2024014）;建设面向南亚东南亚科技创新中心专项项目（202403AP140013）