目的 研究三七Panax notoginseng花中的非皂苷类化学成分及其抗氧化和抗炎活性。方法 采用65%乙醇水溶液热回流提取，石油醚、醋酸乙酯与正丁醇萃取，通过RP-18、硅胶、MCI gel CHP20P、Sephadex LH-20等柱色谱及半制备液相色谱等方法进行化合物的分离及纯化，综合运用核磁共振谱、高分辨质谱、紫外、计算ECD等技术进行结构鉴定，并对部分化合物进行初步的抗氧化活性和抗炎活性评价。结果 从三七花醋酸乙酯部分分离纯化得到7个非皂苷类化合物，包括1个异香豆素类（1）、4个简单酚类（2～5）和2个黑麦草内酯类化合物（6、7）。分别鉴定为异香豆素F1（1）、阿魏酸（2）、evofolin A（3）、对羟基苯甲酸（4）、4-羟基苯甲醛（5）、异黑麦草内酯（6）和黑麦草内酯（7）。结论 化合物1～7均为首次从三七中分离得到，其中化合物1为新的异香豆素，命名为异香豆素F1；化合物2有较强的DPPH自由基清除能力。

Objective To study the non-saponin chemical constituents of the flower buds of Panax notoginseng and evaluate their antioxidant and anti-inflammatory activities. Methods The extraction was performed by refluxing with 65% aqueous ethanol, followed by successive liquid-liquid extraction with petroleum ether, ethyl acetate (EtOAc) and n-butanol. The compounds were isolated and purified using RP-18, silica gel, MCI gel CHP20P, Sephadex LH-20 column chromatography and semi-preparative liquid chromatography. Structural identification was carried out by comprehensive analysis of nuclear magnetic resonance (NMR) spectroscopy, high-resolution mass spectrometry (HR-MS), ultraviolet (UV) spectroscopy, and computational electronic circular dichroism (ECD) spectroscopy. Some purified compounds were evaluated for preliminary antioxidant activity and anti-inflammatory activity. Results A total of seven non-saponin components were isolated from the EtOAc fraction of P. notoginseng flower buds, including one isocoumarin (1), four simple phenols (2—5), and two loliolides (6, 7), were obtained from the EtOAc fraction. These were identified as isocoumarin F1 (1), ferulic acid (2), evofolin A (3), p-hydroxybenzoic acid (4), 4-hydroxybenzaldehyde (5), (＋)-epiloliolide (6), and (-)-loliolide (7), respectively. Conclusion All compounds (1—7) were isolated from P. notoginseng for the first time. Of which, compound 1 is a new isocoumarin and named isocoumarin F1, while compound 2 exhibited obvious DPPH radical scavenging ability.

R284.1

云南省科技厅重点研发计划(202203AC100008);植物化学与西部植物资源持续利用国家重点实验室课题(P2022-KF15)