目的 研究麒麟竭Daemonorops draco果实的化学成分及其心肌细胞保护活性。方法 采用硅胶、ODS、Sephadex LH-20凝胶柱色谱以及半制备高效液相色谱技术进行分离、纯化，通过其理化性质及UV、IR、HRESIMS、NMR等谱学数据鉴定化合物的结构。采用缺糖缺氧（oxygen-glucosedeprivation，OGD）损伤的H9c2心肌细胞模型对分离得到的化合物进行心肌损伤保护活性筛选。结果 从麒麟竭果实乙醇提取物醋酸乙酯部位中共分离鉴定了3对新黄烷类化合物，分别为 (2S)-2-乙氧基-7-羟基-5-甲氧基-6-甲基黄烷（1a）、(2R)-2-乙氧基-7-羟基-5-甲氧基-6-甲基黄烷（1b）、(2S,3S)-2-乙氧基-3,7-二羟基-5-甲氧基-6-甲基黄烷（2a）、(2R,3R)-2-乙氧基-3,7-二羟基-5-甲氧基-6-甲基黄烷（2b）、(2R,3S)-2-乙氧基-3,7-二羟基-5-甲氧基-6-甲基黄烷（3a）和 (2S,3R)-2-乙氧基-3,7-二羟基-5-甲氧基-6-甲基黄烷（3b）。化合物1b和2b在20 μmol/L的浓度下对OGD损伤的H9c2细胞存活率分别提高了（6.36±1.26）%和（10.78±0.76）%。结论 化合物1a/1b、2a/2b和3a/3b为3对对映异构体，均为新化合物，分别命名为麒麟竭素I₁、麒麟竭素I₂、麒麟竭素J₁、麒麟竭素J₂、麒麟竭素K₁和麒麟竭素K₂，其中化合物1b和2b有一定的保护心肌细胞损伤活性。

Objective To investigate the chemical constituents and their myocardial cell protective activity from the fruits of Daemonorops draco. Methods Compounds were isolated and purified using a combination of silica gel, RP-C₁₈, Sephadex LH-20 and semi-preparative HPLC. Their structures were elucidated through comprehensive spectroscopic analysis, UV, IR, HRESIMS and NMR. The protective effects of all isolates on the H9c2 myocardial cells injury induced by oxygen-glucose deprivation (OGD) were evaluated. Results Three pairs of new flavans were purified and elucidated as (2S)-2-ethoxy-7-hydroxy-5-methoxy-6-methylflavan (1a), (2R)-2-ethoxy-7-hydroxy-5-methoxy-6-methylflavan (1b), (2S,3S)-2-ethoxy-3,7-dihydroxy-5-methoxy-6-methylflavan (2a), (2R,3R)-2-ethoxy-3,7-dihydroxy-5-methoxy-6-methylflavan (2b), (2R,3S)-2-ethoxy-3,7-dihydroxy-5-methoxy-6-methylflavan (3a) and (2S,3R)-2-ethoxy-3,7-dihydroxy-5-methoxy-6-methylflavan (3b). Compounds 1b and 2b demonstrated protective effects against OGD-induced H9c2 cell injury, which increased cell viability by (6.36 ±1.26)% and (10.78 ±0.76)% at a concentration of 20 μmol/L, respectively. Conclusion Compounds 1a/1b, 2a/2b, and 3a/3b are three pairs of enantiomers, and they are all new compounds, named as dracoropin I₁, dracoropin I₂, dracoropin J₁, dracoropin J₂, dracoropin K₁, dracoropin K₂. Among them, compounds 1b and 2b exhibited protective effects on myocardial cells.

R284.1

国家自然科学基金重点项目(82030114);国家中医药管理局青年岐黄学者培养项目([2022]256)