目的 建立一种快速鉴别蜘蛛香V. jatamansi和混淆品欧细辛A. europaeum、细辛A. heterotropoides的特异性聚合酶链式反应（PCR）方法。方法 通过Mega 6.0软件对蜘蛛香、欧细辛和细辛的ITS2序列进行对比，寻找差异位点设计特异性引物，优化PCR反应条件，并对该方法的专属性和适用性进行考察。结果 蜘蛛香特异性PCR鉴别方法，采用引物对F-T/R-T、退火温度58 ℃、循环次数28次，经PCR扩增和凝胶电泳后蜘蛛香在约151 bp处得到特异性条带，而混淆品无此条带，此外该方法最低可检测出DNA质量浓度为0.1 ng/µL的样品。结论 所建立的特异性PCR方法可准确、快速对蜘蛛香及其混淆品进行鉴别，为维药蜘蛛香的用药安全提供保障。

Objective To establish a specific polymerase chain reaction (PCR) method for the rapid identification of Valeriana jatamansi and its adulterants Asarum europaeum and Asarum heterotropoides. Methods The ITS2 sequences of V. jatamansi, A. europaeum and A. heterotropoides were compared by Mega 6.0 software to find differential sites to design specific primers, optimise the PCR reaction conditions and examine the specificity and applicability of the method. Results V. jatamansi specific PCR identification method, using primer pair F-T/R-T, annealing temperature of 58 ℃, the number of cycles 28 times, after PCR amplification and gel electrophoresis V. jatamansi specific bands at about 151 bp, while the adulterants have no bands, in addition, the method can detect samples with a minimum DNA concentration of 0.1 ng/µL. Conclusion The established specific PCR method can accurately and rapidly identify V. jatamansi and its adulterants, and provide a guarantee for the safety of the medication of traditional Uighur V. jatamansi.

R286.2

“天山英才”-青年拔尖人才项目（2022TSYCCX0022）；新疆维吾尔自治区重点研发项目（2022B03007-2）；国家中医药管理局项目（GZY-KJS-2024-012）