目的 研究海洋中药羊栖菜Sargassum fusiforme的化学成分。方法 采用硅胶色谱、ODS、MCI柱色谱及半制备高效液相色谱等手段进行分离纯化，采用HR-ESI-MS、1D和2D NMR及文献比对等方法对所获得化合物进行结构鉴定，通过电子圆二色谱（electrostatic circular dichroism，ECD）确定新化合物的绝对构型。结果 从羊栖菜全草50%乙醇提取物中分离得到14个化合物，分别鉴定为 (23E)-3β-羟基豆甾-5,23二烯-7,28-二酮（1）、(24R)-5,28-豆甾二烯-3β,24-二醇（2）、(24S)-5,28-豆甾二烯-3β,24-二醇（3）、24R,28R-和24S,28S-环氧-24-乙基胆甾醇（4）、3-羟基胆甾-5-烯-24-酮（5）、植醇（6）、apo-9¢-fucoxanthinone（7）、loliolide acetate（8）、5-羟基-3,4-二甲基-5-丙基呋喃-2(5H)-酮（9）、herbidosporayilanensin I（10）、丁二酰亚胺（11）、3-羟基吡啶（12）、5-乙氧基甲基尿嘧啶（13）和棕榈酸（14）。结论 化合物1为新化合物，命名为马尾藻甾醇C（sargassuol C）；化合物9～12为首次从马尾藻属中分离得到；化合物8为首次从羊栖菜中得到。

Objective To study the chemical constituents from marine Chinese medicine Sargassum fusiforme. Methods The extract was separated and purified by silica gel column chromatography, octadecylsilyl silica (ODS), MCI column chromatography and semi-preparative high-performance liquid chromatography (HPLC). The structures of the compounds were determined based on High Resolution Electrospray Mass Spectrometry (HR-ESI-MS), 1D and 2D Nuclear Magnetic Resonance (NMR) spectroscopy and comparison with literature data. The absolute configuration of new compound was determined by electrostatic circular dichroism (ECD) calculations. Results A total of fourteen compounds were isolated from the 50% ethanol extract of S. fusiforme, and they were illustrated as (23E)-3β-hydroxy-stigmasta-5,23-dien-7,28-dione (1), (24R)-5,28-stigmastadien-3β,24-diol (2), (24S)-5,28-stigmastadien-3β,24-diol (3), 24R,28R-and 24S,28S-epoxy-24-ethylcholesterol (4), 3-hydroxy-cholest-5-en-24-one (5), phytol (6), apo-9¢-fucoxanthinone (7), loliolide acetate (8), 5-hydroxy-3,4-dimethyl-5-propylfuran-2(5H)-one (9), herbidosporayilanensin I (10), succinimide (11), 3-hydroxypyridine (12), 5-ethoxymethyl uracil (13) and hexadecanoic acid (14). Conclusion Compound 1 is a new compound, which is named sargassuol C, 9—12 are firstly obtained from genus of Sargassum, and 8 is isolated from S. fusiforme for the first time.

R284.1

广东省自然资源厅“海洋六大产业”专项重点项目（粤自然资合[2024]25）