[关键词]
[摘要]
目的 采用智能感官结合化学计量学方法对春秋两季节采收肉苁蓉Cistanche deserticola进行品质评价,为肉苁蓉的生产和质量控制提供参考。方法 利用色差仪测定肉苁蓉样品的色度值,利用电子鼻测定肉苁蓉的气味,采用HPLC测定肉苁蓉的环烯醚萜和苯乙醇苷含量,最后测定其多糖含量,采用皮尔逊相关性分析、正交-偏最小二乘法判别分析等方法进行综合分析,比较春季和秋季采收肉苁蓉的质量。结果 秋季和春季采收肉苁蓉药材气味差异主要在氮氧化合物和萜烯类成分。颜色上春季采收肉苁蓉的色度值L*和b*高于秋季,说明春季的明亮色和黄色高于秋季,秋季采收肉苁蓉颜色更深。质地上秋季采收肉苁蓉硬度值显著低于春季,秋季采收肉苁蓉质地更为柔软;苯乙醇苷类和环烯醚萜类含量二者差异显著,秋季采收肉苁蓉的京尼平苷酸、马钱苷酸、松果菊苷、肉苁蓉苷A和毛蕊花糖苷含量均显著高于春季,分别是春季采收肉苁蓉的2.1、4.1、3.1、3.4、1.4倍。多糖含量为秋季采收肉苁蓉低于春季,但二者差异不显著。结论 秋季采收肉苁蓉较春季在颜色上更深,质地上更柔软,主要活性成分苯乙醇苷类和环烯醚萜类成分含量更高。研究结果可为不同季节采收肉苁蓉的品质评价提供依据。
[Key word]
[Abstract]
Objective To evaluate the quality of Cistanche deserticola harvested in spring and autumn using intelligent sensory combined with chemometrics methods, providing a reference for the production and quality control of C. deserticola. Methods The chromaticity values of C. deserticola samples were measured using a colorimeter, the odor was determined using an electronic nose, and the contents of iridoids and phenylethanoid glycosides were assayed by HPLC. The polysaccharide content was also determined. Comprehensive analysis was performed using Pearson correlation analysis, orthogonal partial least squares discriminant analysis, and other methods to compare the quality of C. deserticola harvested in spring and autumn. Results The primary differences in odor between C. deserticola harvested in autumn and spring were in nitrogen oxides and terpene components. In terms of color, the L* and b* values of spring-harvested C. deserticola were higher than those of autumn-harvested samples, indicating brighter and more yellow hues in spring compared to the darker color of autumn-harvested samples. In terms of texture, the hardness value of autumn-harvested C. deserticola was significantly lower than that of spring-harvested samples, suggesting a softer texture in autumn. Significant differences were also observed in the contents of phenylethanoid glycosides and iridoids, with autumn-harvested samples showing significantly higher levels of geniposidic acid, loganic acid, echinacoside, cistanoside A, and verbascoside, which were 2.1, 4.1, 3.1, 3.4, and 1.4 times higher, respectively, than those in spring-harvested samples. The polysaccharide content of autumn-harvested C. deserticola was lower than that of spring-harvested samples, but the difference was not significant. Conclusion C. deserticola harvested in autumn is darker in color and softer in texture compared to spring, and has a higher content of the main active components, phenylethanol glycosides and iridoids. This study provides a reference for quality evaluation of C. deserticola harvested in different seasons.
[中图分类号]
R286.12
[基金项目]
甘肃省自然科学基金项目(23JRRA1213);甘肃省科技重大专项(23ZDFA013-1);中药保障与创新能力提升项目(甘中医药综函〔 2024〕 14号);甘肃省高校中(藏)药化学与质重点实验室开放基金资助项目(zzy-2024-05);中国工程院战略研究咨询项目(GS2021ZDA06);甘肃省珍稀中药资源评价与保护利用工程研究中心开放基金资助项目(GSZXZY202203)