目的 探究牛膝炭纳米类成分（Achyranthis Bidentatae Radix Carbonisatum nano-components，ABRC-NCs）对酒精性肝病（alcoholic liver disease，ALD）的保护作用及潜在机制。方法 从牛膝炭中提取分离出ABRC-NCs，通过CCK-8实验评估其安全性，利用纳米材料表征方法分析其形态结构、光学性质和官能团性质等。将36只雄性C57BL/6J小鼠随机分为对照组、模型组、联苯双酯（450 mg/kg）组及ABRC-NCs高、中、低剂量（5.00、2.50、1.25 mg/kg）组，每组6只，连续给药6 d。末次给药2 h后ig 50%乙醇溶液造模，每12小时ig 1次，连续2次，构建急性ALD小鼠模型。造模结束后观察肝组织形态及病理变化；检测血清中丙氨酸氨基转移酶（alanine aminotransferase，ALT）、天冬氨酸氨基转移酶（aspartate aminotransferase，AST）活性及肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白细胞介素-6（interleukin-6，IL-6）、IL-1β、IL-10水平；检测肝组织中超氧化物歧化酶（superoxide dismutase，SOD）活性及丙二醛（malondialdehyde，MDA）、谷胱甘肽（glutathione，GSH）、三酰甘油（triglyceride，TG）水平；采用免疫组化法检测肝组织核因子E2相关因子（nuclear factor E2-related factor 2，Nrf2）、血红素氧合酶-1（heme oxygenase-1，HO-1）蛋白表达。结果 3.91～1 000.00 μg/mL的ABRC-NCs对RAW264.7细胞无毒性作用。ABRC-NCs为近球形结构，表面含有丰富的官能团。体内实验结果表明，ABRC-NCs可减轻ALD小鼠肝组织损伤，显著降低血清中ALT、AST活性及促炎因子TNF-α、IL-6、IL-1β水平（P＜0.01），升高抗炎因子IL-10水平（P＜0.01），提高肝组织抗氧化酶SOD活性及GSH水平（P＜0.01），下调肝组织MDA和TG水平（P＜0.01），同时上调肝组织Nrf2、HO-1蛋白表达（P＜0.05、0.01）。结论 ABRC-NCs可能通过抑制炎症反应、减轻氧化应激损伤，对急性ALD发挥保护作用，为牛膝炭的临床应用及ALD的临床治疗提供新思路。

Objective To explore the protective effect and potential mechanism of Achyranthis Bidentatae Radix Carbonisatum nano-components (ABRC-NCs) on alcoholic liver disease (ALD). Methods ABRC-NCs were extracted from Achyranthis Bidentatae Radix Carbonisatum and their safety was evaluated by CCK-8 test; The morphological structure, optical properties and functional group properties were analyzed by characterization method of nanomaterials. A total of 36 male C57BL/6J mice were randomly divided into control group, model group, bifendate (450 mg/kg) group, ABRC-NCs high-, medium- and low-dose (5.00, 2.50, 1.25 mg/kg) groups, with six mice in each group, mice were administered with drugs for 6 d. An acute ALD mouse model was established by gavage of 50% ethanol solution 2 h after the last administration, with gavage every 12 h for 2 consecutive times. The morphology and pathological changes of liver tissue were observed after modeling. The activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as well as the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β and IL-10 in serum were detected; The activity of superoxide dismutase (SOD) and the levels of malondialdehyde (MDA), glutathione (GSH), triglycerides (TG) in liver tissue were detected ; Immunohistochemistry was used to detect the protein expressions of nuclear factor E2 related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in liver tissue. Results ABRC-NCs at concentrations of 3.91—1 000.00 μg/mL had no toxic effect on RAW264.7 cells. ABRC-NCs had a nearly spherical structure with abundant functional groups on their surface. The in vivo experimental results showed that ABRC-NCs could alleviate liver tissue damage in ALD mice, significantly reduce activities of ALT, AST and levels of pro-inflammatory factors TNF-α, IL-6 and IL-1β in serum (P < 0.01), increase anti-inflammatory factor IL-10 level (P < 0.01), increase antioxidant enzyme SOD activity and GSH level in liver tissue (P < 0.01), down-regulate levels of MDA and TG in liver tissue (P < 0.01), and up-regulate Nrf2 and HO-1 protein expressions in liver tissue (P < 0.05, 0.01). Conclusion ABRC-NCs may exert a protective effect on acute ALD by inhibiting inflammatory response and reducing oxidative stress damage, providing new ideas for the clinical application of Achyranthis Bidentatae Radix Carbonisatum and the clinical treatment of ALD.

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