目的 研究女娄菜Silene aprica中黄酮类化学成分及其抗炎活性。方法 用80%乙醇提取药材，聚酰胺富集总黄酮，利用常压柱色谱色谱及高压制备液相色谱进行分离，通过化合物的理化性质及现代波谱技术对相关化合物进行结构表征，采用CCK-8法评价化合物的细胞毒性，并利用脂多糖诱导RAW 264.7小鼠单核巨噬细胞体外炎症模型进行抗炎活性评价。结果 从女娄菜总黄酮部分分离得到11个黄酮类化合物，分别为herbcetin-3-O-β-D-(2-O-β-D-glucopyranoside)-7-O-β-D-glucopyranoside（1）、草质素-3-槐二糖-8-葡萄糖苷（2）、棉花皮素-3-槐二糖-8-葡萄糖苷（3）、槲皮素-3-槐二糖-7-葡萄糖苷（4）、草质素苷（5）、肥皂草苷（6）、夏佛塔苷（7）、异荭草苷（8）、异牡荆苷（9）、异金雀花素（10）、槲皮素（11）。体外抗炎活性评价中，化合物1～9均对NO生成有一定的抑制作用。结论 化合物1为新的黄酮苷类化合物，命名为女娄菜苷M；化合物2～6为首次从该植物中分离得到；药理活性表明，化合物1～3能显著的降低脂多糖诱导RAW264.7小鼠单核巨噬细胞NO的释放，其半数抑制浓度（median inhibition concentration，IC₅₀）在4.15～10.82 μmol/L。

Objective To investigate of the flavonoids components and anti-inflammatory activity of Silene aprica. Methods The medicinal materials were extracted with 80% ethanol and enrich total flavonoids with polyamide. Silica gel, Sephadex LH-20 and Pre-HPLC were used to isolate and purify these compounds. The structures of the related compounds were characterized based on their physicochemical properties and modern spectroscopic techniques such as NMR, MS, and so on. The cytotoxicity of the obtained compounds was screened using the CCK-8 method, and evaluating the anti-inflammatory activity through the RAW264.7 mouse mononuclear macrophages induced by lipopolysaccharide (LPS). Results A total of 11 flavonoids compounds were isolated and identified asherbcetin-3-O-β-D-(2-O-β-D-glucopyranoside)-7-O-β-D-glucopyranoside (1), herbacetin-3-β-D-(2-O-β-D-glucopyrano- sidoglucopyranoside)-8-β-D-glucopyranoside (2), gossypetin-3-β-D-(2-O-β-D-glucopyranosidoglucopyranoside)-8-β-D-glucopyra- noside (3), quercetin-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl-7-O-β-D-glucopyranoside (4), herbacetin 7-rhamnoside (5), saponarin (6), schaftoside (7), isoorientin (8), isovitexin (9), isoscoparin (10), quercetin (11), respectively. In the experiment of anti-inflammatory activities evaluation, all compounds had inhibitory effect on NO production. Conclusion Compound 1 is a new flavonoid glycoside compound named sileneside M, and 2—6 were isolated from this plant for the first time. In addition, the in vitro activities of the compounds were determined by using the LPS induced RAW264.7 cell inflammation model, with Dexamethasone as the positive control. The bioactivity results showed that compounds 1—3 have significant anti-inflammatory activity, with IC₅₀ values of 4.15—10.82 μmol/L.

R284.1

四川省自然科学基金项目（2023NSFSC1274）；国家重点研发计划课题（2019YFC1712503）；西南民族大学青藏高原研究科技创新团队（2024CXTD22）；四川省大学生创新训练计划（S202414389077）