目的 建立不同产地北沙参Glehnia littoralis的HPLC指纹图谱及多成分含量测定方法，结合化学模式识别法评价不同产地北沙参药材质量。方法 采用haloC₁₈（150 mm×4.6 mm，2.7μm）色谱柱，以乙腈（B）-0.1%乙酸水溶液（A）为流动相，柱温40 ℃，体积流量0.4 mL/min，检测波长254 nm，绘制23批不同产地的北沙参药材的指纹图谱，应用SPSS 27.0和SIMCA18软件结合化学模式识别，聚类分析（clusteranalysis，CA）、主成分分析（principal component analysis，PCA）及正交偏最小二乘法判别分析（orthogonal partial least squares discriminant analysis，OPLS-DA）对不同产地北沙参样品进行质量评价，并测定10种主要成分的含量。结果 23批北沙参HPLC指纹图谱共匹配出22个共有峰，分别指认出芦丁、异槲皮素、槲皮素、补骨脂素、花椒毒素、佛手柑内酯、欧前胡素、异欧前胡素、人参二醇、法卡林二醇10个成分；指纹图谱相似度在0.698～0.999，CA将23批北沙参分为3类；PCA与OPLS-DA结果为产于山东胡成、河北安国、山东牟平的药材质量较好；并分析筛选出人参二醇、法卡林二醇、花椒毒素、佛手柑内酯、欧前胡素、异欧前胡素和补骨脂素7个成分为引起不同产地质量差异的标志性成分。北沙参中10个主要成分芦丁、异槲皮素、槲皮素、补骨脂素、花椒毒素、佛手柑内酯、欧前胡素、异欧前胡素、人参二醇、法卡林二醇的质量分数分别为4.75～32.96、1.12～20.42、6.06～22.85、0.93～24.15、2.24～36.46、8.12～59.57、2.77～27.39、10.05～44.67、75.19～524.35、96.73～654.32μg/g。结论 建立的北沙参HPLC指纹图谱操作简便、结果可靠，人参二醇、法卡林二醇、花椒毒素、佛手柑内酯、欧前胡素、异欧前胡素、补骨脂素和异槲皮素可以作为北沙参质量评价的主要指标性成分。

Objective To establish the HPLC fingerprint and multi-component content determination method of Glehnia littoralis combined with chemical pattern recognition method and evaluate the quality of G. littoralis from different Genuine producing areas. Methods The HPLC fingerprint of 23 batches of G. littoralis from different Genuine producing areas was established using a haloC₁₈column (150 mm × 4.6 mm, 2.7 μm), with acetonitrile (B) and 0.1% acetic acid aqueous solution (A) as the mobile phase, column temperature at 40 ℃, flow rate at 0.4 mL/min, and detection wavelength at 254 nm. The quality of G. littoralis samples from different Genuine producing areas was evaluated by cluster analysis (CA), principal component analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS-DA) using SPSS 27.0 and SIMCA 18 software, and the contents of 10 main components were determined. Results A total of 22 common peaks were matched in the HPLC fingerprints of 23 batches of G. littoralis, and 10 components were identified as rutinum, isoquercitrin, quercetin, psoralen, 8-methoxypsoralen, bergapten, imperatorin, isoimperatorin, Panaxydiol(1, 8-heptadecadiene-4, 6-diacetylene-3, 10-diol), and Falcarindiol (1,9-heptadecadiene-4,6-diyne-3,8-diol). The similarity of the fingerprints was between 0.698 and 0.999. The 23 batches of G. littoralis were classified into three categories by cluster analysis. The results of PCA and OPLS-DA analysis indicated that the quality of G. littoralis from Hu Cheng, Shandong, An Guo, Hebei, and Mu Ping, Shandong was better. Seven components, including panaxydiol, falcarindiol, 8-methoxypsoralen, bergapten, imperatorin, isoimperatorin and psoralen,were identified as the marker components causing the quality differences among different producing areas. The contents of 10 main components in G. littoralis, including rutinum, isoquercitrin, quercetin, psoralen, 8-methoxypsoralen, bergapten, imperatorin, isoimperatorin, panaxydiol, and falcarindiol, were 4.75—32.96, 1.12 —20.42, 6.06—22.85, 0.93—24.15, 2.24—36.46, 8.12—59.57, 2.77—27.39, 10.05—44.67, 75.19—524.35, and 96.73—654.32 μg/g, respectively. Conclusion The established HPLC fingerprint of G. littoralis is simple and reliable, and1, 8-heptadecadiene-4, 6-diacetylene-3, 10-diol, 1,9-Heptadecadiene-4,6-diyne-3,8-diol, 8-methoxypsoralen, bergapten, imperatorin, isoimperatorin psoralen and isoquercitrincan be used as the main index components for the quality evaluation of G. littoralis.

R286.2

山东中医药科技面上项目（M-2022166）；2023年全省中医药高层次人才培育项目（项目编号：1）；国家中医药管理局科技司-山东省卫生健康委员会共建中医药科技项目（GZY-KJS-SD-2023-051）；药品生产技术专业群建设（2023XDRHXMXK07）