目的 建立白薇Cynanchi Atrati Radix高效液相色谱（high performance liquid chromatography，HPLC）指纹图谱和多成分含量测定方法，结合化学模式识别评价其质量。方法 采用Waters-Symmetry-C₁₈（250 mm×4.6 mm，5 µm）色谱柱，以乙腈-0.1%磷酸水为流动相梯度洗脱，体积流量为1.0 mL/min，柱温为25℃，进样量为20 µL，采集波长为270、310 nm。通过中药指纹图谱相似度分析软件及化学模式识别对26批白薇饮片进行分析，并同时采用外标法测定其中尿苷、对羟基苯乙酮和2,4-二羟基苯乙酮含量。结果 26批白薇饮片指纹图谱标定了14个共有峰，确认了其中3个共有峰，分别为尿苷（5号峰）、对羟基苯乙酮（9号峰）、2,4-二羟基苯乙酮（12号峰），相似度为0.626～1.000；依据聚类分析（cluster analysis，CA）、主成分分析（principal component analysis，PCA）和偏最小二乘法-判别分析（partial least squares-discriminant analysis，OPLS-DA）结果可将26批白薇饮片分为2大类，筛选出4个主成分，累积贡献率80.652%。白薇饮片中尿苷、对羟基苯乙酮和2,4-二羟基苯乙酮质量分数分别为0.14～0.29、1.74～2.74、0.25～0.49 mg/g。结论 建立的白薇HPLC指纹图谱分析操作简单、结果可靠，为白薇资源及其相关产品的品质评价提供了依据。

Objective A high performance liquid chromatography (HPLC) fingerprint and multi-components content determination method was established quality control of Baiwei (Cynanchi Atrati Radix et Rhizoma) combined with chemical pattern recognition. Methods The HPLC method was performed on an Agilent 1200 series instrument equipped with a Waters-Symmetry-C₁₈ (250 mm × 4.6 mm) column employing a gradient elution procedure with acetonitrile and 0.1% phosphoric acid in water as the mobile phase. The working parameters were set as follows: the volume flow rate at 1.0 mL/min, the column temperature at 25 ℃, the injection volume at 20 μL, and the acquisition wavelengths at 270 nm and 310 nm, respectively. Totally 26 batches of Cynanchi Atrati Radix et Rhizoma decoction pieces were analyzed by fingerprint similarity analysis software and chemical pattern recognition, and the contents of uridine, p-hydroxyacetophenone and 2,4-dihydroxyacetophenone were simultaneously determined by external standard method. Results The fingerprints of 26 batches of Cynanchi Atrati Radix et Rhizoma decoction pieces were characterized and 14 common peaks were pointed out, three of which were identified as uridine (peak 5), p-hydroxyacetophenone (peak 9), and 2,4-dihydroxyacetophenone (peak 12), with the similarity ranging from 0.626 to 1.000, respectively. Through cluster analysis (CA), principal component analysis (PCA) and partial least squares-discriminant analysis (OPLS-DA), the 26 batches of Cynanchi Atrati Radix et Rhizoma samples can be classified into two major groups, and four principal components were screened out, with a cumulative contribution rate of 80.652%. The contents of uridine, p-hydroxyacetophenone and 2,4-dihydroxyacetophenone in the tested decoction pieces were ranged from 0.14 mg/g to 0.29 mg/g, from 1.74 mg/g to 2.74 mg/g and from 0.25 mg/g to 0.49 mg/g, respectively. Conclusion The established HPLC fingerprint analytical method for qualitative and quantitative control of Cynanchi Atrati Radix et Rhizoma is simple and reliable, which could provide a basis for the quality evaluation of Cynanchi Atrati Radix et Rhizoma resources and the related products.

R286.2

国家自然科学基金资助项目（81973211/C0033375）；湖南省自然科学基金资助项目（2024JJ8168）；2023年度国家药品标准修订研究课题（2023Z03）；湖南中医药大学“十四五”重点学科-生物工程学科（校行发规字［2023］2号）