目的 克隆连翘Forsythia suspensa的FsADH1基因，初步探究蛋白定位以及在低温胁迫下该基因的表达模式。方法 参考连翘转录组数据，克隆得到FsADH1基因，并通过生物信息学方法对其编码蛋白的理化性质进行分析，利用烟草叶片瞬时转化分析蛋白亚细胞定位、实时荧光定量PCR（qRT-PCR）技术分析在低温胁迫下的表达模式。结果 连翘FsADH1基因长度为1 143 bp，编码380个氨基酸，为亲水性蛋白，在连翘幼苗根、茎、叶中均有表达。FsADH1基因编码的蛋白定位在细胞质中，低温胁迫下，连翘幼苗FsADH1基因表达量呈先上升后下降的趋势，第3天达到最高值。结论 FsADH1基因受到低温胁迫显著诱导，其可能参与连翘低温胁迫响应调控，为后续研究FsADH1基因功能以及提高连翘抗寒能力的研究提供一定的参考基础。

Objective Cloning of the FsADH1 gene from Forsythia suspensa, and a preliminary exploration of the protein localization and the expression pattern of this gene under low-temperature stress. Methods Based on the transcriptome data of F. suspensa, the FsADH1 gene was cloned. The physicochemical properties of the protein encoded by this gene were analyzed using bioinformatics methods. The sub-cellular localization of the protein was analyzed through transient transformation in Nicotiana tabacum leaves, and the expression pattern under low-temperature stress was analyzed by real-time fluorescence quantitative PCR (qRT-PCR) technology. Results The FsADH1 gene was 1 143 bp in length and encoded 380 amino acids, which was a hydrophilic protein, which was expressed in the roots, stems and leaves of F. suspensa seedlings. The protein encoded by FsADH1 gene was localized in the cytoplasm, and the expression level of FsADH1 gene in F. suspensa seedlings increased first and then decreased under low temperature stress, reaching the maximum value on the third day. Conclusion FsADH1 gene is significantly induced by low temperature stress and may be involved in the regulation of F. suspensa low temperature stress response. This provides a certain reference basis for the subsequent studies on the function of the FsADH1 gene and the improvement of the cold resistance of F. suspensa.

R286.12

国家自然科学基金项目（U1404829）；河南省自然科学基金项目（202102110156）；河南省中央引导地方发展资金项目（Z20241471030）；

河南省中药材产业科技特派员服务团项目；河南省科技攻关项目（242102110325）