目的 炮附片-山茱萸为临床救治心衰病常用药对，首次分析药对“炮附片-山茱萸”的物质基础，为其临床应用提供实验数据。方法 采用超高效液相色谱四极杆/飞行时间质谱（UPLC-Q-TOF-MS/MS）和化学成分数据库匹配对药对炮附片-山茱萸进行全成分分析。同时将药对（炮附片-山茱萸）与单味药（炮附片、山茱萸）化学成分进行对比和归类分析。结果 通过与各单味药炮附片、山茱萸化学成分数据库进行出峰的保留时间、准分子离子峰和二级质谱数据等信息对比分析，从药对水提液中共鉴定到114个化学成分；其中64个生物碱成分与炮附片相关：双酯型二萜类生物碱10个、长链脂型二萜类生物碱4个、单酯型二萜类生物碱19个、无酯型二萜类生物碱30个、多酯型二萜类生物碱1个；50个成分与山茱萸相关：环烯醚萜类成分19个、黄酮及其苷类成分10个、鞣质类成分6个、芳香酚酸类成分7个、三萜类成分2个、其他类成分6个。对比分析发现炮附片相关成分在药对中减少59个；而山茱萸中相关成分在药对中减少37个。结论 首次对炮附片-山茱萸药对进行全成分分析，从成分分析角度探究炮附片-山茱萸药对配伍规律。同时对比分析了药对炮附片-山茱萸与单味药炮附片、山茱萸的差异性成分，为其临床合理应用奠定物质基础。

Objective The drug pair of PAC (Paofupian, the process of Aconiti Lateralis Radix Praeparata) and Cor (Shanzhuyu, Corni Fructus) is one of the important drug pairs to treat heart failure of traditional Chinese medicine in clinical, this article firstly analyzed the material basis of drug-pair PAC-Cor to provide experimental data for its clinical application. Methods The full component analysis of the drug-pair PAC-Cor was conducted using an ultra-high performancce liquid chromatography with quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) and chemical composition database. Meanwhile, the chemical compositions of drug-pair (PAC-Cor) and the single herbs (PAC, Cor) were compared and classified. Results Comprehensive comparing and analyzing the retention time of each peak, quasi-molecular ion peak and secondary mass spectrum data of the PAC and Cor, 114 chemical components were firstly analyzed from the water extract of drug-pair PAC-Cor. Among them, 64 alkaloids were associated with PAC: 10 diester diterpenoid alkaloids (DDAs), four lipo-alkaloids (Lipo-As), 19 monoester diterpenoid alkaloids (MDAs), 30 ester-free diterterpenoid alkaloids (ADAs), and one polyester diterpenoid alkaloid (PDAs). A total of 50 components were related to the Cor: 19 iridoids (Irs), 10 flavonoids and their glycosides (Fls), six tannins (Tas), seven aaromatic phenolic acids (APAs), two triterpenoids (Trs), and six other compounds (Ots). Through comparing with PAC, the numbers of compounds decreased 59 in drug pair. Meanwhile, the numbers of compounds associated with Cor similarly reduced 37. Conclusion A full component analysis was conducted on the drug-pair PAC-Cor for the first time, and the compatibility rules of the drug-pair PAC-Cor were explored from the perspective of component analysis. Moreover, differential compounds among thedrug-pair of PAC-Cor and single herbs (PAC, Cor) were compared and analyzed to lay a material foundation for its rational clinical applications.

R284.1

广东省中医药局科研项目（20251169）；广州中医药大学“高水平大学建设”项目（No.81）；广州中医药大学“薪火计划”重点项目（XH20170110）