目的 研究刺茶美登木Maytenus variabilis的倍半萜生物碱类化学成分及其细胞毒活性。方法 运用正相硅胶柱色谱、制备薄层色谱及制备型HPLC等方法进行化合物分离纯化，并用 1D NMR、2D NMR、HRMS等现代波谱技术进行化合物的结构鉴定。采用MTT法研究化合物的抗肿瘤活性。结果 从刺茶美登木中分离鉴定2个倍半萜生物碱类化合物，分别鉴定为：(1R,2S,4S,5S,6R,7R,9R,10R)-1,2-dinicotinoyloxy-4-hydroxy-6,15-diacetoxy-9-benzoyloxy-dihydro-β-agarofuran（1）和 (1R,2S,4S,5S,6R,7R,9R,10R)-1,6,15-triacetoxy-2,9-dinicotinoyloxy-4-hydroxy-dihydro-β-agarofuran（2）。结论 化合物1和2为新的倍半萜生物碱类化合物，分别命名为美登木宁碱A及美登木宁碱B。细胞毒活性研究显示，化合物1和2对人胃癌HGC-27细胞有一定的体外增殖抑制活性，其半数抑制浓度（median inhibition concentration，IC₅₀）分别为69.44、65.74 μmol/L。

Objective To study the sesquiterpene alkaloids from Maytenus variabilis and their cytotoxic activities. Methods The compounds were isolated and purified by column chromatography over normal phase silica gel, preparative thin-layer chromatography and preparative HPLC. Their structures were elucidated by means of various spectroscopic techniques including one-dimensional nuclear magnetic resonance (1D NMR), two-dimensional nuclear magnetic resonance (2D NMR) and high resolution mass spectrometry (HRMS). The anti-tumor activity of the compound was investigated by MTT assay. Results Two sesquiterpene alkaloids were isolated from M. variabilis, their structures were elucidated as (1R,2S,4S,5S,6R,7R,9R,10R)-1,2-dinicotinoyloxy-4-hydroxy-6,15-diacetoxy-9-benzoyloxy-dihydro-β-agarofuran (1) and (1R,2S,4S,5S,6R,7R,9R,10R)-1,6,15-triacetoxy-2,9-dinicotinoyloxy-4-hydroxy-dihydro-β-agarofuran (2), respectively. Conclusion The cytotoxic activity study revealed that compounds 1 and 2 exhibited certain in vitro proliferation inhibitory activity against human gastric cancer HGC-27 cell with IC₅₀values of 69.44, 65.74 μmol/L.

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湖北省中央引导地方科技发展资金项目（2024BSB016）；三峡大学人才科研启动基金项目（8253302）