目的 研究裸花紫珠Callicarpa nudiflora地上部分95%乙醇提取物正丁醇部位的化学成分及其体外抗炎活性。方法 采用硅胶、ODS、Sephadex LH-20和高效液相等多种色谱分离技术进行分离纯化，通过理化性质和波谱数据鉴定化合物结构，新化合物的绝对构型则采用电子圆二色谱（electronic circular dichroism，ECD）确定。结果 从裸花紫珠正丁醇部位分离得到9个化合物，分别鉴定为 (3S,7S)-n-butyl tuberonate-12-O-[6'-O-(E)-feruloyl]-β-D-glucopyranoside（1）、syringaresinol-4'-O-D-monoglucopyranoside（2）、杜仲脂素A（3）、毛蕊花糖苷（4）、异毛蕊花糖苷（5）、木犀草素-7-O-β-D-葡萄糖苷（6）、木犀草素-4'-O-β-D-葡萄糖苷（7）、(7R)-hydroxyeucommic acid n-butyl ester（8）、2-phenylethyl-β-D-glucopyranoside（9）。结论 化合物1为新化合物，命名为裸花紫珠苷E；化合物8为首次从裸花紫珠分离得到。体外抗炎实验表明，化合物1、3～5、7～9均有不同程度的抗炎活性。

Objective To investigate the chemical constituents of the n-butanol fraction from the 95% ethanol extracts of Callicarpa nudiflora and evaluate their in vitro anti-inflammatory activity. Methods A variety of chromatographic techniques, including silica gel column chromatography, ODS, Sephadex LH-20, and HPLC, were employed for the isolation and purification of the compounds. Their structures were elucidated based on spectroscopic data and physicochemical properties, while the absolute configurations of the new compound was determined using electronic circular dichroism (ECD). Results A total of nine compounds were isolated from the n-butanol fraction of C. nudiflora, which were identified as (3S,7S)-n-butyl tuberonate-12-O-[6'-O-(E)-feruloyl]-β-D-glucopyranoside (1), syringaresinol-4'-O-D-monoglucopyranoside (2), eucommin A (3), verbascoside (4), isoverbascoside (5), luteolin 7-O-β-D-glucoside (6)、luteolin-4'-O-β-D-glucopyranoside (7), (7R)-hydroxyeucommic acid n-butyl ester (8)、2-phenylethyl-β-D-glucopyranoside (9). Conclusion Compound 1 is a new compound named nudifloside E. Compound 8 was isolated from C. nudiflora for the first time. In vitro anti-inflammatory assays demonstrated that compounds 1, 3—5, 7—9 had anti-inflammatory activities in varying degrees.

R284.1

福建省自然科学基金项目（2022J05326）；广东省自然科学基金项目（2023A1515011761）；深圳市“医疗卫生三名工程”项目资助（SZZYSM202206005）