目的 为了解类萌发素蛋白（germin-like protein，GLP）在人参Panax ginseng中的功能，对人参GLP基因(PgGLPs)家族进行了鉴定，明确其在不同类型组织材料的表达模式。方法 利用生物信息学分析了PgGLPs的染色体定位、基因结构、保守基序、进化关系、表达模式和顺式作用元件等。同时基于以上分析结果选取4条PgGLP基因研究其在人参种胚、愈伤组织、实生苗根、叶柄、叶不同组织材料中的表达情况。结果 在人参转录组数据库中鉴定得到28条PgGLP转录本，染色体定位结果表明PgGLPs不均匀地分布在人参12条染色体上。系统发育分析将PgGLPs分为3个亚型，分别包含3、10、15个成员，其中亚型Ⅱ中只包含PgGLPs。顺式作用元件预测结果显示，PgGLPs启动子区域存在多种与植物激素、生长发育、逆境胁迫相关响应元件。PgGLP成员在人参不同组织部位中的表达水平差异较大，4条PgGLPs在人参种胚的表达普遍高于其他组织部位，具有组织特异性，其中PgGLP16-01在不同组织部位中的表达量高于其他3条基因。结论 鉴别出28个人参PgGLP家族成员，其表达呈现组织特异性，PgGLP基因家族的系统鉴定及表达分析为人参生长发育相关基因家族的研究奠定理论基础，为进一步研究植物类萌发素蛋白提供参考。

Objective To investigate the function of germin-like proteins in Panax ginseng, the P. ginseng GLP gene family was characterized to clarify its expression pattern in different types of tissue materials. Methods The chromosomal localisation, gene structure, conserved motifs, evolutionary relationships, expression patterns and cis-acting elements of PgGLPs were analysed using bioinformatics. Based on the above analysis, four PgGLP genes were selected to study their expression in six different tissue materials: P. ginseng embryos, adventitious roots, healing tissues, roots of living seedlings, petioles and leaves. Results In this study, 28 PgGLP transcripts were identified in the P. ginseng transcriptome database, and chromosomal localisation results showed that PgGLPs were unevenly distributed on 12 chromosomes of P. ginseng. Phylogenetic analysis classified PgGLPs into three isoforms containing 3, 10 and 15 members, respectively, with only PgGLPs in isoform II. cis-acting element prediction showed that there were several response elements in the promoter region of PgGLPs related to phytohormones, growth and development, and abiotic stress.The expression levels of PgGLP members varied widely in different tissue parts of P. ginseng, and four PgGLPs were found in different tissue parts of P. ginseng. PgGLPs were generally expressed at higher levels in P. ginseng seed embryos than in other tissues with tissue specificity, among which the expression of PgGLP16-01 was higher than that of the other three genes in different tissues. Conclusion In this study, 28 members of the P. ginseng PgGLP family were identified and their expression showed tissue specificity. The systematic identification and expression analysis of the P. ginseng PgGLP gene family provides a theoretical basis for the study of P. ginseng growth and development related gene families and a reference for the further study of plant germin-like proteins.

R286.12

吉林省科技厅项目（20240101227JC）