目的 建立甘草干姜汤（Gancao Ganjiang Decoction，GGD）的HPLC指纹图谱及多成分测定分析方法，为GGD质量评价提供依据。方法 采用HPLC法，色谱柱Hubble-C₁₈柱（250 mm×4.6 mm，5 µm）；以乙腈-0.05%磷酸水溶液为流动相，梯度洗脱；柱温30 ℃；体积流量0.8 mL/min；检测波长230 nm，对15批GGD进行指纹图谱测定，并结合中药色谱指纹图谱相似度评价系统、聚类分析（cluster analysis，CA）、主成分分析（principal component analysis，PCA）对15批GGD样品进行全面分析评价。以乙腈-0.05%磷酸溶液为流动相，梯度洗脱；体积流量1.0 mL/min；柱温为35 ℃；检测波长230 nm；对芹糖甘草苷、甘草苷、甘草素、甘草酸、6-姜烯酚多成分进行定量测定。结果 建立了GGD HPLC指纹图谱，有14个共有峰，指纹图谱相似度＞0.998；通过对照品指认8个特征性成分，分别为芹糖甘草苷、甘草苷、芹糖异甘草苷、异甘草苷、甘草素、甘草酸、6-姜酚、6-姜烯酚；当平方欧式距离为10时，15批样品通过聚类分析分成2类。定量测定中芹糖甘草苷、甘草苷、甘草素、甘草酸、6-姜烯酚进样量在76.5～1 529.1、25.7～514.1、10.3～206.6、229.4～4 587.8、21.8～435.7 ng线性关系良好（r＝0.999 1）；平均回收率在96.0%～102.2%，RSD均＜2%；15批GGD中芹糖甘草苷、甘草苷、甘草素、甘草酸、6-姜烯酚的质量分数分别为2.27～5.60、1.12～2.70、0.29～0.72、9.13～15.43、0.16～0.46 mg/g。结论 建立的HPLC指纹图谱及定量测定方法适用于GGD的全面质量评价，该方法操作简便、准确可靠、重复性好，为GGD的质量控制提供了有效手段。

Objective To establish HPLC fingerprint and the multi-component content determination method of Gancao Ganjiang Decoction (GGD, 甘草干姜汤), providing a basis for quality evaluation of GGD. Methods HPLC was used with a Hubble-C₁₈ column (250 mm×4.6 mm, 5 μm); Acetonitrile-0.05% phosphoric acid solution was used as the mobile phase for gradient elution; The column temperature was 30 ℃; The flow rate was 0.8mL/min; The detection wavelength was 230 nm. The fingerprint spectra of 15 batches of GGD were determined, and a comprehensive analysis and evaluation of the 15 batches of GGD samples were conducted using a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, cluster analysis (CA), and principal component analysis (PCA). The content determination of multiple components including liquiritin apioside, liquiritin, liquiritigenin, glycyrrhizic acid, and 6-shogaol was carried out using acetonitrile-0.05% phosphoric acid solution as the mobile phase with gradient elution; The flow rate was 1.0 mL/min; The column temperature was 35 ℃; The detection wavelength 230 nm. Results The fingerprint spectrum of GGD was established, and a total of 14 common peaks were matched, with a fingerprint similarity greater than 0.998. A total of eight characteristic components were identified by reference standards, including liquiritin apioside, liquiritin, isoliquiritin apioside, isoliquiritin, liquiritigenin, glycyrrhizic acid, 6-gingerol, and 6-shogaol. When the squared Euclidean distance was 10, the 15 batches of samples were divided into two categories by clustering analysis. In the content determination, the inlet quantity of liquiritin apioside, liquiritin, liquiritigenin, glycyrrhizic acid and 6-shogaol had good linear relationships (r = 0.999 1) in the range of 76.5—1 529.1, 25.7—514.1, 10.3—206.6, 229.4—4 587.8, 21.8—435.7 ng. The average recovery rate was 96.0%—102.2%, and RSD < 2%. The mass fractions of liquiritin apioside, liquiritin, liquiritigenin, glycyrrhizic acid and 6-shogaol were 2.27—5.60, 1.12—2.70, 0.29—0.72, 9.13—15.43 and 0.16—0.46 mg/g, respectively. Conclusion The established fingerprint spectrum and content determination method are suitable for the comprehensive quality evaluation of GGD. This method is easy to operate, accurate, reliable, and has good repeatability, providing an effective means for the quality control of GGD.

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