目的 研究昆仑雪菊Coreopsis tinctoria花的化学成分，并对分离到的化合物进行抗炎活性研究。方法 使用AB-8大孔吸附树脂粗分，制备液相对各粗分段进行精细分离得到单体化合物，采用波谱技术鉴定化合物结构；并采用脂多糖（lipopolysaccharides，LPS）诱导RAW 264.7细胞建立的炎症模型对化合物抗炎活性进行筛选。结果 从昆仑雪菊醋酸乙酯萃取部位分离到13个黄酮类化合物，分别鉴定为 (2R)-黄诺马苷（1）、(2S)-黄诺马苷（2）、花旗松素-7-O-β-D-吡喃葡萄糖苷（3）、紫铆黄素-7-O-β-D-葡萄糖苷（4）、栎草亭-7-O-β-D-吡喃葡萄糖苷（5）、gossypetin-5-O-(6''-(E)-caffeoyl)-b-D-glucoside（6）、紫铆因-4'-O-β-D-吡喃葡萄糖苷（7）、槲皮素-7-O-b-D-吡喃葡萄糖苷（8）、奥卡宁-4'-O-b-D-吡喃葡萄糖苷（9）、8,3',4'-三羟基黄酮-7-O-b-D-葡萄糖苷（10）、异奥卡宁（11）、奥卡宁（12）、栎草亭（13）。Griess实验显示化合物4～6、9、11、12对RAW264.7炎症模型细胞NO产生显著的抑制作用。结论 化合1、2、6和13为首次从昆仑雪菊中分离得到的化合物；化合物4～6、9、11、12具有一定的抗炎活性。

Objective To study chemical constituents of the flower of Coreopsis tinctoria and investigate the anti-inflammatory activity of these compounds. Methods Separation and purification were carried out using AB-8 macroporous adsorption resin, the monomer compounds were obtained by fine separation of each coarse segment by preparative liquid phase and the structure of compounds were identified using spectroscopic techniques. RAW 264.7 inflammatory model cells induced by lipopolysaccharides (LPS) were used to screen the anti-inflammatory activity of compounds. Results A total of 13 flavonoids were isolated from the ethyl acetate extract of C. tinctoria and identified as (2R)-flavanomarein (1), (2S)-flavanomarein (2), taxifolin-7-O-b-D-glucopyranoside (3), butin-7-O-b-D-glucoside (4), quercetagetin-7-O-b-D-glucopyranoside (5), gossypetin-5-O-(6''-(E)-caffeoyl)-b-D-glucoside (6), butein-4'-O-b-D-glucopyranoside (7), quercetin-7-O-b-D-glucopyranoside (8), okanin-4'-O-b-D-glucopyranoside (9), 8,3',4'-trihydroxyflavone-7-O-b-D-glucoside (10), isookanin (11), okanin (12), 3,3',4',5,6,7-hexahydroxyflavone (13). Griess experiments showed that compounds 4—6, 9, 11 and 12 have inhibitory effects on NO production in RAW264.7 inflammatory model cells. Conclusion Compound 1, 2, 6 and 13 are isolated for the first time from C. tinctoria; Compound 4—6, 9, 11 and 12 exhibit anti-inflammatory activity.

R284.1

青海省科技厅基础研究项目-昆仑雪菊抗炎活性成分及机制研究（2022-ZJ-730）