目的 克隆山茱萸Cornus officinalis G10H1基因（CoG10H1），并进行相关的生物信息学分析、表达分析和亚细胞定位研究。方法 基于山茱萸转录组数据筛选并克隆CoG10H1基因，并对其CoG10H1及其编码蛋白的理化性质、二级结构、三级结构等进行分析。利用实时荧光定量聚合酶链式反应PCR（real-time fluorescent quantitative polymerase chain reaction，qRT-PCR）对CoG10H1进行组织特异性表达分析。构建pCAMBIA1300-CoG10H1-GFP融合表达载体并侵染烟草叶片，激光共聚焦显微镜观察CoG10H1蛋白的亚细胞定位。结果 CoG10H1的cDNA长度为1 497 bp，编码499个氨基酸，有1个信号肽，在茎中表达量稍高，果实和叶片中次之，激光共聚焦显微镜下观察到CoG10H1蛋白定位于内质网中。结论 CoG10H1蛋白属于细胞色素P450蛋白家族，可能在马钱苷合成通路中的发挥关键作用，为后续研究CoG10H1基因在马钱苷合成通路调控中的作用研究提供参考。

Objective The CoG10H1 gene was cloned from Cornus officinalis and analyzed by bioinformatics, expressionand subcellular localization.Methods The CoG10H1 gene was screened and cloned based on the transcriptome data of C. officinalis, and the physicochemical properties, secondary structure, and tertiary structure of CoG10H1 and its encoded protein were analyzed. The tissue-specific expression analysis of CoG10H1 was conducted by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR). The pCAMBIA1300-CoG10H1-GFP fusion expression vector was constructed and infected with tobacco leaves, and the subcellular localization of the CoG10H1 protein was observed using a laser confocal microscope. Results The cDNA length of CoG10H1 was 149 7 bp, encoding 499 amino acids, with one signal peptide. The expression level was slightly higher in the stem, followed by the fruit and the leaf. The CoG10H1 protein was observed to be located in the endoplasmic reticulum under the laser confocal microscope. Conclusion The CoG10H1 protein belongs to the Cytochrome P450 protein family and may play a crucial role in the synthesis pathway of loganin, providing a reference for subsequent studies on the role of the CoG10H1 gene in the regulation of the loganin synthesis pathway.

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国家自然科学基金项目（U1404829）；中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”（2060302）；河南省科技攻关项目（242102110325）；河南省中药材产业科技特派员服务团项目；中央引导地方科技发展资金项目（Z20241471030）