目的 探索忍冬Lonicera japonica基因（LjMLO14）抗白粉病机制。方法 应用病毒诱导的基因沉默技术（virus induced gene silencing，VIGS）抑制忍冬LjMLO14基因的表达，通过接种白粉菌进行抗性鉴定。结果 研究发现，在VIGS载体转化忍冬15 d后，LjMLO14基因的相对表达量显著下降了80%～90%，沉默效果显著。对基因沉默后的忍冬叶片进行白粉病抗性鉴定，菌丝体的生长较野生型显著减弱，抗性水平较野生型显著提高。结论 为初步鉴定LjMLO14基因对忍冬白粉病的调控功能，培育忍冬抗白粉病新品种提供理论依据。

Objective To explore the mechanism of Lonicera japonica gene (LjMLO14) against powdery mildew.Methods In this study, virus induced gene silencing (VIGS ) was used to suppress the expression of the LjMLO14 gene in L. japonica, and the resistance was identified by inoculating powdery mildew. Results Fifteen days after VIGS vector transformation, the relative expression of the LjMLO14 gene was significantly reduced by 80%—90% compared with the control group, indicating a robust silencing effect. The powdery mildew resistance of L. japonica leaves after gene silencing was identified. The growth of mycelium was significantly weaker than that of the wild type, and the resistance level was significantly higher than that of wild type. Conclusion This study provides a theoretical basis for the preliminary identification of the regulatory function of LjMLO14 gene on powdery mildew of L. japonica and the cultivation of new varieties of L. japonica resistant to powdery mildew.

R286.12

山东省重点研发计划（乡村振兴科技创新提振行动计划）项目（2022TZXD0036）；山东省农业良种工程项目（2021LZGC008）；山东省现代农业产业技术体系中草药创新团队项目（SDAIT-20）