目的 对黄腺香青Anaphalis aureopunctata的化学成分及活性进行研究。方法 采用Sephadex LH-20柱色谱，正、反相硅胶柱色谱以及半制备高效液相色谱等分离方法，纯化分离得到化合物，通过理化性质鉴别、NMR、MS等手段鉴定其结构。通过抗菌和抗氧化评价所得化合物的活性。结果 从中分离得到6个化合物，分别鉴定为5-羟基-6-(3-甲基-2-丁烯基)-7-O-β-D-葡萄糖苷-1(3H)-异苯并呋喃酮（1）、5,7-二羟基-1(3H)-异苯并呋喃酮（2）、platypterophthalide（3）、7-O-β-D-葡萄糖基阔翼内酯（4）、香青醇（5）、anaphalisol 7-O-β-D-glucopyranoside（6）。抗菌活性表明，6个化合物对2种革兰阳性菌的抑制作用更为明显；抗氧化活性表明，化合物1、2、4、6对ABTS自由基的半数抑制浓度（median inhibition concentration，IC₅₀）值分别为59.81、76.02、65.01、77.23 μmol/L。结论 化合物1和4为新化合物，分别命名为香青异戊烯苷（1）和香青阔翼内酯葡萄糖苷（4）；化合物6为首次从香青属中分离得到，化合物2、3、5和6为首次从该植物中得到，所有化合物对2种革兰阳性菌有一定的抑制效果，化合物1、2、4、6表现出良好的抗氧化活性。

Objective To study the chemical constituents and bioactivities of Anaphalis aureopunctata. Methods Compounds were isolated through chromatographic techniques, including Sephadex LH-20 column chromatography, normal and reversed-phase silica gel column chromatography, and semi-preparative HPLC. The structures of the isolated compounds were elucidated by means of physicochemical properties and spectral analyses (NMR, MS). The antimicrobial and antioxidant activities of the compounds were evaluated. Results Six compounds were isolated and identified as 5-hydroxy-6-(3-methyl-2-butenyl)-7-O-β-D-glucopyranosyl-1(3H)-isobenzofuranone (1), 5,7-dihydroxy-1(3H)-isobenzofuranone (2), platypterophthalide (3), 7-O-β-D-glucosyl- platypterophthalide (4), anaphaliol (5), and anaphalisol 7-O-β-D-glucopyranoside (6). Antimicrobial activity assays revealed that the six compounds exhibited more pronounced inhibitory effects against the two Gram-positive bacterial strains. Antioxidant activity assays indicated that the IC₅₀ values of compounds 1, 2, 4, and 6 against ABTS radicals were 59.81, 76.02, 65.01, and 77.23 μmol/L. Conclusion New compounds 1 and 4 were named as anaphaloprenoside (1) and anaphalopteroside (4). Compounds 6 were isolated from the genus Anaphalis for the first time. Compounds 2, 3, 5, and 6 were obtained from this plant for the first time. All six compounds showed certain inhibitory effects against the two Gram-positive bacteria, and compounds 1, 2, 4, and 6 demonstrated good antioxidant activities.

R284.1

国家自然科学基金青年基金资助项目（82204237）；天然产物研究与利用湖北省重点实验室（2024NPRD01，2024NPRD06）