目的 构建白芷Angelicae Dahuricae Radix药材、饮片、标准汤剂、流浸膏剂及血清样本的HPLC特征图谱，并依据其质量传递过程，筛选白芷的质量标志物（quality markers，Q-Marker）。方法 收集10批白芷药材，并加工成饮片、标准汤剂、流浸膏剂和血清样本。采用HPLC技术，构建5类样本的特征图谱，通过相似度评价和指标成分的含量及转移率分析，筛选白芷的Q-Marker。结果 白芷样本（药材、饮片、标准汤剂、流浸膏剂及血清）的特征图谱指认出8个高相似度的特征峰。在药材至饮片传递中，7种关键成分（欧前胡素、佛手柑内酯、白当归素、异欧前胡素、蛇床子素、水合氧化前胡素、精氨酸）的平均转移率超61%，传递稳定；饮片至标准汤剂传递中，香豆素类成分水合氧化前胡素、白当归素、佛手柑内酯可以稳定传递（＞60%），但精氨酸转移率异常高（＞700%），而欧前胡素、异欧前胡素的含量低且转移率＜20%，蛇床子素未检出；标准汤剂至流浸膏剂传递中，精氨酸、水合氧化前胡素、白当归素稳定传递（＞70%）；流浸膏剂至血清样本传递中，仅精氨酸、水合氧化前胡素、白当归素检出并传递，其余成分未检出。结论 结合白芷药材、饮片、标准汤剂、流浸膏剂和血清样本的特征图谱、含量及转移率变化，筛选出水合氧化前胡素和白当归素可作为白芷的Q-Marker。

Objective To establish the HPLC fingerprint profiles of Baizhi (Angelicae Dahuricae Radix, ADR) medicinal materials, decoction pieces, standard decoctions, fluid extracts, and serum samples, and screen quality markers (Q-Marker) of ADR based on its quality transfer process. Methods A total of ten batches of ADR medicinal materials were collected and processed into decoction pieces, standard decoctions, fluid extracts, and serum samples. The fingerprint profiles of the five types of samples were established by using HPLC technology. The Q-Marker of ADR were screened through similarity evaluation and analysis of the content and transfer rates of indicator components. Results A total of eight high-similarity characteristic peaks were identified in the fingerprint profiles of ADR samples (medicinal materials, decoction pieces, standard decoctions, fluid extracts, and serum). During the transfer from medicinal materials to decoction pieces, the average transfer rates of seven key components (imperatorin, bergapten, byakangelicin, isoimperatorin, osthole, oxypeucedanin hydrate, and arginine) exceeded 61%, indicating stable transfer. In the transfer from decoction pieces to standard decoctions, the coumarin components oxypeucedanin hydrate, byakangelicin, and bergapten were stably transferred (> 60%). However, the transfer rate of arginine was abnormally high (> 700%), while the content of imperatorin and isoimperatorin was low with transfer rates < 20%, and osthole was not detected. During the transfer from standard decoctions to fluid extracts, arginine, oxypeucedanin hydrate, and byakangelicin were stably transferred (> 70%). In the transfer from fluid extracts to serum samples, only arginine, oxypeucedanin hydrate, and byakangelicin were detected and transferred, while the other components were not detected. Conclusion Based on the fingerprint profiles, content, and transfer rate changes of ADR medicinal materials, decoction pieces, standard decoctions, fluid extracts, and serum samples, oxypeucedanin hydrate and byakangelicin were screened as Q-Marker of ADR.

R283.6

国家自然科学基金资助项目（31860102）；甘肃省委组织部陇原青年创新创业人才（个人）项目（GSRC-2023-1-4）；甘肃省高校教师创新基金项目（2023A-53）