目的 基于温胆汤指纹图谱和指标成分定量测定，结合化学计量学方法分析其共煎传统汤剂（traditional decoction，TD）与单煎混合TD化学成分的差异，为温胆汤等方药科学制备和质量控制提供参考。方法 采用HPLC法建立温胆汤10批共煎TD与10批单煎混合TD的指纹图谱，并评价其相似度；测定温胆汤中腺苷、辛弗林、甘草苷、柚皮苷、橙皮苷、6-姜酚和环磷酸腺苷7种指标成分的含量；采用主成分分析（principal component analysis，PCA）、多维标度分析（multidimensional scaling analysis，MDSA）和正交偏最小二乘法-判别分析（orthogonal partial least squares discriminant analysis，OPLS-DA）对指标成分进行数据分析。结果 以10批温胆汤共煎TD的指纹图谱生成共有模式图谱，对照共有模式图谱，共煎TD与单煎混合TD的指纹图谱相似度分别为相似度1：0.897±0.043、0.845±0.069（n＝10，P＜0.01），相似度2：0.842±0.083、0.759±0.274（n＝10，P＞0.05）；通过指纹图谱及指标成分定量测定发现，温胆汤共煎TD与单煎混合TD在化学成分种类上未见明显差异，含量上温胆汤共煎TD中腺苷、辛弗林、甘草苷、橙皮苷、6-姜酚的含量高于单煎混合TD（P＜0.01），分别为单煎混合TD的2.037、1.926、2.505、4.265、1.964倍，柚皮苷、环磷酸腺苷含量与单煎混合TD无显著性差异（P＞0.05）；PCA、MDSA和OPLS-DA结果一致，可将温胆汤共煎TD与单煎混合TD分为2类。结论 所建立的HPLC指纹图谱结合PCA、MDSA和OPLS-DA及多成分定量测定能客观、有效地显示温胆汤共煎TD与单煎混合TD中主要成分的差异，可为温胆汤质量控制和临床合理用药提供参考。

Objective Based on the fingerprint and quantitative determination of index components of Wendan Decoction, combined with chemometrics methods, the differences of chemical components between co-decoction traditional decoction (TD) and single decoction mixed TD were analyzed, which provided a reference for the scientific preparation and quality control of Wendan Decoction and other prescriptions. Methods The fingerprints of 10 batches of co-decocted TD and 10 batches of single-decocted mixed TD of Wendan Decoction were established by HPLC, and their similarities were evaluated. The contents of adenosine, synephrine, liquiritin, naringin, hesperidin, 6-gingerol and adenosine cyclophosphate in Wendan Decoction were determined. Principal component analysis (PCA), multidimensional scaling analysis (MDSA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to analyze the data of the index components. Results The fingerprint of 10 batches of Wendan Decoction co-decoction TD was used to generate a common pattern map. Compared with common pattern map, the similarity of fingerprint of co-decoction TD and single decoction mixed TD was similarity 1: 0.897 ± 0.043 and 0.845 ± 0.069 (n = 10, P < 0.01); similarity 2: 0.842 ± 0.083, 0.759 ± 0.274 (n = 10, P > 0.05). Through the fingerprint and quantitative determination of index components, it was found that there was no significant difference in the types of chemical components between the co-decoction TD and the single-decoction mixed TD. The contents of adenosine, synephrine, liquiritin, hesperidin and 6-gingerol in the co-decoction TD were higher than those in the single-decoction mixed TD (P < 0.01). They were 2.037, 1.926, 2.505, 4.265 and 1.964 times of single decoction mixed TD, respectively. There was no significant difference in the content of naringin and adenosine cyclophosphate between co-decoction TD and single decoction mixed TD (P > 0.05). The results of PCA, MDSA and OPLS-DA were consistent, and the Wendan Decoction co-decoction TD and single decoction mixed TD could be divided into two categories. Conclusion The established HPLC fingerprint combined with PCA, MDSA, OPLS-DA and multi-component quantitative determination can objectively and effectively show the difference of main components in Wendan Decoction co-decoction TD and single decoction mixed TD, which can provide reference for quality control and clinical rational drug use of Wendan Decoction.

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河南省自然科学基金项目杰出青年科学基金项目（242300421023）；河南省卫生健康中青年学科带头人专项（HNSWJW-2020014）；河南省卫生健康委国家中医临床研究基地科研专项（2021JDZY036）；2022年青年岐黄学者培养项目（No.［2022］256）；河南省卫生健康委国家中医临床研究基地科研专项（2021JDZY014）