目的 研究湖北金粟兰Chloranthus henryi var. hupehensis的化学成分及其抗炎活性。方法 采用硅胶、凝胶、HPLC等色谱技术进行提取分离与纯化，运用MS、NMR、ECD等波谱方法并结合文献数据，对化合物结构进行鉴定，采用脂多糖（lipopolysaccharides，LPS）诱导的鼠巨噬细胞RAW 264.7体外炎症模型进行抗炎活性评价。结果 从该植物根部分离得到11个倍半萜类化合物，分别鉴定为4β,5α,9α-trihydroxy-7-oxoguaia-8(11)-en-12,9-olid（1）、8S,9S-环氧化白术内酯II（2）、6β,10α-dihydroxy-1-oxoeremophila-7(11),8(9)-dien-12,8-olide（3）、长尾粗叶木内酯A（4）、6α-hydroxyeudesma-4(15),7(11),8(9)-triene-12,8-olide（5）、白术内酯III（6）、chlorantene C（7）、chlomultin B（8）、dihydrocurcolone（9）、commiphorene B（10）、5β(H)-10α-methyl-8-oxoeudesma-4(15),7(11)-dien-12,6-olide（11）。结论 化合物1为新的愈创木烷型倍半萜，命名为湖北金粟兰内酯N，化合物2～3、9～11为首次从金粟兰属植物中分离得到，化合物1～3、6～11为首次从湖北金粟兰中分离得到。化合物2对LPS诱导的鼠巨噬细胞RAW 264.7的一氧化氮（NO）产生有一定抑制作用，半数抑制浓度（median inhibition concentration，IC₅₀）为（29.0±11.3）μmol/L。

Objective To study the chemical constituents and anti-inflammatory activities of Chloranthus henryi var. hupehensis. Methods The compounds were extracted, isolated, and purified by column chromatography of silica gel, gel and HPLC, and their structures were identified using spectroscopic methods such as MS, NMR, and ECD combined with literature data. The in vitro anti-inflammatory activities of all compounds were evaluated using the lipopolysaccharide (LPS) induced inflammatory model of RAW 264.7 mouse macrophage cells. Results A total of 11 sesquiterpenoids were isolated from the roots of C. henryi var. hupehensis, which were identified as 4β,5α,9α-trihydroxy-7-oxoguaia-8(11)-en-12,9-olid (1), 8S,9S-epoxylatractylenolide II (2), 6β,10α-dihydroxy-1-oxoeremophila-7(11),8(9)-dien-12,8-olide (3), lasianthuslactone A (4), 6α-hydroxyeudesma-4(15),7(11),8(9)-triene-12,8-olide (5), atractylenolide Ⅲ (6), chlorantene C (7), chlomultin B (8), dihydrocurcolone (9), commiphorene B (10), 5β(H)-10α-methyl-8-oxoeudesma-4(15),7(11)-dien-12,6-olide (11). Conclusion Compound 1 was a new guaiane-type sesquiterpene named chlorahupelactone N, while compounds 2—3, 9—11 were isolated from Chloranthus genus for the first time, and compounds 1—3, 6—11 were isolated from C. henryi var. hupehensis for the first time. Compound 2 inhibited the production of nitric oxide (NO) in LPS-induced RAW 264.7 mouse macrophage cells with a median inhibition concentration (IC₅₀) value of (29.0 ± 11.3) μmol/L.

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河北省自然科学基金资助项目（H2022209046）；中央引导地方科技发展基金项目（246Z2506G）；南京市中医药科技专项基金资助项目（ZYYB202203）