目的 研究粗糠柴Mallotus philippensis果实的化学成分。方法 采用硅胶、ODS、凝胶及半制备高效液相等色谱分离技术进行分离纯化，通过质谱、核磁共振波谱及圆二色谱等方法对化合物进行结构鉴定，采用肉汤微量稀释法评价化合物的抗菌活性。结果 从粗糠柴果实的甲醇提取物中分离得到6个化合物，分别鉴定为粗糠柴黄酮P（1）、粗糠柴黄酮Q（2）、5-羟基-7-甲氧基二氢黄酮（3）、(2S)-7-hydroxy-5-methoxy-8-prenylflavanone（4）、(2R,3S,4R)-2,3,4-trimethyl-3-[(3E,7E,11E)-3,8,12,16-tetramethyl-3,7,11,15-heptadecatetraen-1-yl]cyclohexanone（5）和豆甾-4-烯-3-酮（6）。化合物1对新型隐球菌的最低抑菌浓度（minimum inhibitory concentration，MIC）值为128 μg/mL。结论 化合物1和2为新化合物，化合物5为新天然产物，化合物3和4为首次从大戟科植物中分离得到，化合物6为首次从野桐属植物中分离得到。化合物1对新型隐球菌有一定的抑制作用。

Objective To investigate the chemical constituents of the fruit of Mallotus philippensis. Methods The compounds were separated and purified by a variety of chromatographic methods including silica gel ODS, gel and semi-preparative high performance liquid chromatography. Their structures were established based on mass spectrometry, nuclear magnetic resonance spectroscopy and circular dichroism. The antimicrobial activities of these isolates were evaluated by broth microdilution method. Results A total of six compounds were obtained from the methanol extract of the fruit of M. philippensis and identified as mallophilol P (1), mallophilol Q (2), 5-hydroxy-7-methoxyflavanone (3), (2S)-7-hydroxy-5-methoxy-8-prenylflavanone (4), (2R,3S,4R)-2,3,4-trimethyl-3-[(3E,7E,11E)-3,8,12,16-tetramethyl-3,7,11,15-heptadecatetraen-1-yl]cyclohexanone (5) and stigmast-4-en-3-one (6), respectively. The minimum inhibitory concentration (MIC) value of compound 1 against Cryptococcus neoformans was 128 μg/mL. Conclusion Compounds 1 and 2 are new compounds, compound 5 is a new natural product, compounds 3 and 4 are reported from the Euphorbiaceae plants for the first time, and compound 6 is reported from the genus Mallotus for the first time. Compound 1 shows certain antimicrobial effect against C. neoformans.

R284.1

国家自然科学基金资助项目（82373751）；深圳市博士后留深资助项目