[关键词]
[摘要]
目的 对金果榄Tinospora sagittata外观性状与其主要化学成分间的相关性进行研究,探究“辨状论质”理论在金果榄药材鉴别中的科学性。方法 观察并测量金果榄的形状、颜色等9种性状指标;采用HPLC法检测40批次金果榄中木兰花碱、蜕皮激素、非洲防己碱、药根碱、巴马汀和古伦宾含量;比较金果榄的9种外观性状指标、6种化学成分之间的联系,并对金果榄“状-质”数据进行相关性分析和回归分析。结果 金果榄药材的皱缩程度、药材长宽比、表面颜色、断面颜色、L*、a*值各与6种主要化学成分含量具有显著相关性,而b*值与除蜕皮激素外的5种化学成分均呈极显著相关。结论 金果榄药材表面皱缩程度、表面颜色、断面颜色、短径、长宽比、质量以及L*、a*、b*值均可用于快速判断金果榄药材的品质,其中b*值最为关键且全面,结果可为快速全面评价金果榄药材的质量提供参考。
[Key word]
[Abstract]
Objective To study the correlation between the appearance of Jinguolan (Tinosporae Radix) and its main chemical constituents, and to investigate the scientific validity of the theory of “quality evaluation through morphological identification” in the identification of Tinosporae Radix. Methods Observing and measuring nine kinds of trait indexes, including shape and colour of Tinosporae Radix; useding high performance liquid chromatography (HPLC) to detect the contents of magnolflorine, hydroxyecdysone, columbamine, jatrorrhizine, palmatine and columbin in 40 batches of Tinosporae Radix; comparing the association between the nine kinds of appearance trait indexes and the six kinds of chemical compounds in Tinosporae Radix and making a correlation analysis and regression analysis of the Tinosporae Radix “shape-quality” data.Results The degree of crumpling, herb aspect ratio, surface colour, section colour, L* and a* values of Tinosporae Radix were each significantly correlated with the content of six major chemical constituents, whereas the b* values were highly significantly correlated with all five chemical constituents except hydroxyecdysone. Conclusion The surface crumpling degree, surface colour, section colour, short diameter, aspect ratio, weight and L*, a*, b* values can be used to quickly determine the quality of Tinosporae Radix, among which the b* value is the most critical and comprehensive, and the results of this study can provide a reference for the rapid and comprehensive evaluation of the quality of Tinosporae Radix.
[中图分类号]
R286.2
[基金项目]
四川省科技计划重点研发项目(2020YFN0152);四川省科技厅重点研发计划(重大科技专项)社会发展领域重点研发项目(2022YFS0582);国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-D-202209);成都中医药大学杏林学者青基人才专项(QJRC2022034)