目的 基于转录组数据筛选鉴定分析甘松Nardostachys jatamansi CONSTANS（CO）转录因子家族。方法 运用生物信息学技术对甘松植物的CO转录因子家族成员展开综合分析，深入探究其蛋白的理化特性、基因序列结构、亚细胞位置、导肽和信号肽的预测，以及跨膜结构域的识别。此外，通过构建系统进化树，进一步揭示这些基因家族成员间的进化关系。结果 在甘松的数据库中经过筛选、鉴定，最终得到10个CO转录因子，氨基酸长度为316～440 aa，相对分子质量在35 561.67～48 171.96，理论等电点范围为5.11～8.63，主要在细胞核中表达，均含有B-box和CCT结构域，均不含跨膜结构域和信号肽。构建系统进化树明确了各亚族的成员分布：第Ⅰ亚族包含2个成员，第Ⅱ亚族增至3个成员，而第Ⅲ亚族则有5个成员，这一发现为进一步探讨各亚族间的进化关系及特性差异提供了重要基础。结论 利用了生物信息学的手段，全面鉴定并分析了甘松CO转录因子家族的理化性质、蛋白结构、系统发育关系等，对甘松CO转录因子家族功能的详尽研究，为后续的深入探索提供了宝贵的参考。此外，也为理解甘松药用成分合成的调控机制提供了基础，并为未来培育出更高品质的甘松品种提供了科学依据。

Objective According to the transcriptome data, the CONSTANS (CO) transcription factor family members were identified and analyzed in Nardostachys jatamansi. Methods The bioinformatic technology was used to analyze the CO transcription factor family members of n N. jatamansi plants, including the physicochemical properties of proteins, gene sequence architecture analysis, subcellular location, peptides and signaling peptides prediction, and identification of transmembrane domains. The phylogenetic tree was constructed to reveal the evolutionary relationship between them. Results A total of 10 members of the CO transcription factor family were screened and identified based on the N. jatamansi database. The length of amino acids was 316—440 aa, the relative molecular weight was 35 561.67—48 171.96, and the theoretical range of isoelectric point was 5.11—8.63. They are mainly expressed in the nucleus, contain B-box and CCT domains, and don’t contain transmembrane domains and signal peptides. The phylogenetic tree was constructed to clarify the distribution of members of each subfamily: Subfamily I contains two members, subfamily II has three members, and subfamily III has five members. This discovery provides an important basis for further exploration of the evolutionary relationship and characteristic differences among subfamilies. Conclusion The physicochemical properties, protein structure and phylogenetic relationships of CO family transcription factors were identified and analyzed by means of bioinformatics. The detailed study on the function of CO family transcription factors provided a valuable reference for further exploration. In addition, this study also provides a basis for understanding the regulatory mechanism of medicinal ingredient synthesis of N. jatamansi, and provides a scientific basis for higher-quality breeding in the future.

R286.2

四川省自然科学基金资助项目（2025ZNSFSC1124）;西南民族大学青藏高原研究首批科技创新团队（2024CXTD04）;西南民族大学人才科研项目（RQD2021110）;中央高校基本科研业务费专项基金项目（ZYN2024189）