目的 研究红景天Rhodiola rosea内生真菌 Penicillium thomii C18的次生代谢产物。方法 采用硅胶和Sephadex LH-20 柱色谱及制备型高效液相色谱（HPLC）进行分离纯化，利用核磁共振、质谱、旋光等谱学技术对化合物进行结构鉴定。结果 从Penicillium thomii C18培养物的醋酸乙酯萃取物中共分离得到9个聚酮类化合物，包括1个新的化合物与8个已知化合物，分别鉴定为 (2'S)-5,7-二羟基-2-(2'-羟基-4'-氧代戊基])-色原酮 [(2'S)-5,7-dihydroxy-2-(2'-hydroxy-4'-oxopentyl)-chromone，1]、spirocitromycetin（2）、异决明酮（isotorachrysone，3）、1,6-二羟基-3-甲基-8-甲酯基(口山)酮[1,6-dihydroxy-3-methyl-8-carbomethoxyxanthone，4]、1,5-二羟基-3-甲氧基-7-甲基-蒽-9,10-二酮 (1,5-dihydroxy-3-methoxy-7-methyl-anthracene-9,10-dione，5）、9-O-methylneuchromenin（6）、neuchromenin（7）、星形酸（asterric acid，8）、星形酸甲酯（methyl asterrate，9）。结论 化合物1为新的聚酮类化合物，命名为拖姆酮A，化合物3和5为首次从青霉属中分离得到。

Objective To study the secondary metabolites of the endophyties Penicillium thomii C18 derived from Rhodiola rosea. Methods The secondary metabolites of P. thomii C18 were separated and purified by silica gel, Sephadex LH-20 column chromatography and preparative HPLC. The structures of the isolated compounds were determined by NMR, HR-ESI-MS, polarimetry and etc. Results A total of nine compounds were isolated from the endophytic fungus P. thomii C18. There was one new compound (2'S)-5,7-dihydroxy-2-(2'-hydroxy-4'-oxopentyl)-chromone (1), and eight known compounds spirocitromycetin (2), isotorachrysone (3), 1,6-dihydroxy-3-methyl-8- carbomethoxyxanthone (4), 1,5-dihydroxy-3-methoxy-7-methyl-anthracene-9,10-dione (5), 9-O-methylneuchromenin (6), neuchromenin (7), asterric acid (8), and methyl asterrate (9). Conclusion Compound 1 is a new polyketide named thomiione A. Compounds 3 and 5 are isolated from Penicillium genus for the first time.