目的 优选建曲发酵工艺，为建曲发酵的规范化生产提供参考依据。方法 以橙皮苷、柚皮素、橙皮素、醇溶性浸出物含量、糖化酶活力及外观性状为评价指标，对炮制过程的发酵时间、发酵温度、相对湿度（relative humidity，RH）进行考察，通过AHP-CRITIC综合加权法确定各评价指标的权重系数，联合单因素实验与Box-Behnken设计-响应面法，以综合评分作为评价指标，优选最佳发酵工艺参数。结果AHP-CRITIC综合赋权法确定的橙皮苷、柚皮素、橙皮素、醇溶性浸出物含量及糖化酶活力和外观性状的权重系数分别为0.070 2、0.107 1、0.078 0、0.058 5、0.235 4、0.450 8；优选的建曲发酵工艺为将处方中各中药细粉、麦麸与面粉稀糊揉合均匀并压制成块后，置发酵箱内，在发酵温度35 ℃、RH 80%条件下连续发酵23 h。平行3次的验证试验综合评分均值为87.889，与预测值的相对偏差为1.07%，且各质量指标的RSD均小于3.0%。结论 采用响应面法建立的模型科学准确，优选的建曲发酵工艺稳定可行，可为以建曲为代表的发酵类中药规范化生产和质量标准提高提供参考依据。

Objective To optimize the fermentation process of Jianqu and provide reference for the standardized production of Jianqu fermentation. Methods Using the contents of hesperidin, naringenin, hesperetin and alcohol soluble extract, glucoamylase activity and appearance characteristics. as evaluation indexes, the fermentation time, fermentation temperature and relative humidity (RH) during processing were investigated, and the weight coefficients of each index were determined by AHP-CRITIC hybrid weighting method. Combined with single factor experiment and Box-Behnken design-response surface method, the comprehensive score was used as the evaluation index to optimize the fermentation process parameters. Results The weight coefficients of hesperidin, naringenin, hesperetin and alcohol soluble extract, glucoamylase activity and appearance traits determined by AHP-CRITIC comprehensive weighting method were 0.070 2, 0.107 1, 0.078 0, 0.058 5, 0.235 4 and 0.450 8, respectively. The optimal fermentation process of Jianqu was as follows: the fine powder of traditional Chinese medicine, wheat bran and flour paste in the prescription were evenly kneaded and pressed into blocks, and then placed in a fermentation box for continuous fermentation for 23 h at fermentation temperature of 35 ℃ and fermentation humidity of RH 80%. The average comprehensive score of three parallel verification tests was 87.889, the relative deviation from the predicted value was 1.07%, and the RSD of each quality index was less than 3.0%. Conclusion The model established by response surface method is scientific and accurate, and the optimized fermentation process of Jianqu is stable and feasible, which can provide reference for standardized production and quality standard improvement of fermented Chinese medicines represented by Jianqu.

R283.6

国家重点研发计划“中医药现代化”重点专项（2023YFC3504105）