目的 研究宁夏枸杞Lycium barbarum 叶的化学成分及其体外降糖活性。方法 运用硅胶、MCI、ODS、和Sephadex LH-20等柱色谱以及反相制备型HPLC等各种现代色谱分离技术进行系统的分离纯化。利用波谱学方法确定化合物结构，并采用胰岛素抵抗人肝癌HepG2细胞模型进行体外降糖活性评价。结果 从宁夏枸杞叶95%乙醇提取物的二氯甲烷和醋酸乙酯部位中分离得到17个降倍半萜类化合物，分别鉴定为黑麦草内酯（1）、(3R,6S)-3-hydroxy-2,2,6-trimeth-7-oxabicyclo[4.3.0]non-9-en-8-one（2）、黑麦草素（3）、去氢吐叶醇（4）、3-hydroxy-5,6-epoxy-7-megastigmen-9-one（5）、4,5-dihydroblumenol（6）、蚱蜢酮（7）、3S,5R-dihydroxy-6S,7-megastig-madien-9-one（8）、3,4-dihydroxy-5,7-megastigmadien-9-one（9）、(−)-(3R,4R)-eucomegastigmane B（10）、布卢姆醇B（11）、(6R,9R)-3-酮-α-紫罗兰醇-9-O-β-D-吡喃葡萄糖苷（12）、byzantionoside B（13）、(6S,9S)-roseoside（14）、(6S,9R)-roseoside（15）、staphylionoside D（16）、柑橘苷A（17）。结论 化合物2、3和10为首次从枸杞属中分离得到，化合物2～17为首次从宁夏枸杞叶中分离得到。化合物1～17在10μmol/L浓度时，可以增加胰岛素诱导抵抗HepG2细胞的葡萄糖消耗量。

Objective To investigate the chemical constituents of the leaves of Lycium barbarum and their hypoglycemic activity in vitro. Methods The compounds were isolated and purified by silica gel, MCI, ODS, Sephadex LH-20 column chromatography and reversed phase preparation HPLC. The structures of the compounds were identified by spectroscopic method, and the hypoglycemic activity in vitro was evaluated by insulin resistance HepG2 cell model. Results A total of 17 norsesquiterpenoids were isolated from the dichloromethane and ethyl acetate part of the 95% ethanol extract of the leaves of L. barbarum, and their structures were determined as loliolide (1), (3R,6S)-3-hydroxy-2,2,6-trimeth-7-oxabicyclo[4.3.0]non-9-en-8-one (2), epiloliolide (3), dehydrovomifoliol (4), 3-hydroxy-5,6-epoxy-7-megastigmen-9-one (5), 4,5-dihydroblumenol (6), grasshopper ketone (7), 3S,5R-dihydroxy-6S,7-megastig-madien-9-one (8), 3,4-dihydroxy-5,7-megastigmadien-9-one (9), (−)-(3R,4R)-eucomegastigmane B (10), blumenol B (11), (6R,9R)-3-oxo-α-ionol-9-O-β-D-glucopyranoside (12), byzantionoside B (13), (6S,9S)-roseoside (14), (6S,9R)-roseoside (15), staphylionoside D (16), citroside A (17). Conclusion Compounds 2, 3, and 10 were isolated from Lycium genus for the first time, while compounds 2―17 were isolated from the leaves of L. barbarum for the first time. Meanwhile, compounds 1―17 could increased the glucose consumption in insulin-resistant HepG2 cells at a concentration of 10 μmol/L.

R284.1

国家自然科学基金资助项目（22177027）;河南省高校科技创新团队支持计划（24IRTSTHN039）;河南省科技研发计划联合基金（232301420067）