目的 研究鹰爪花Artabotrys hexapetalus中的化学成分及其细胞毒活性。方法 运用多种色谱方法，对鹰爪花茎和根的95%乙醇提取物进行分离纯化，获得的化合物用核磁共振（NMR）、高分辨质谱（HRESIMS）和电子圆二色谱等手段进行结构鉴定。采用CCK-8法评估化合物对3种肿瘤细胞（人肝癌HepG2细胞、人非小细胞肺癌A549细胞和人结肠癌HCT116细胞）增殖的影响。结果 从鹰爪花95%乙醇提取物中分离得到8个化合物，其中1个新阿朴菲和7个已知的酰胺类生物碱，分别鉴定为(E)-(6aS,7R)-7-羟基-3-甲氧基-N-丙烯醛基-1,2-亚甲二氧基氧化阿朴菲碱（1）、trans-N-p-coumaroyl tyramine（2）、N-trans-3-hydroxy-4-methoxycinna moyltyramine（3）、N-trans-feruloyltyramine（4）、(E)-3-(4-hydroxy-3-methoxyphenyl)-N-phenethyl-acrylamide（5）、northalifoline（6）、tribulusamide A（7）、大麻酰胺F（8）。化合物7和8能显著抑制HepG2细胞增殖，细胞半数抑制浓度（median inhibition concentration，IC₅₀）分别是（30.52±0.93）μmol/L和（16.26±1.97）μmol/L。结论 化合物1为新化合物，命名为鹰爪花碱E（hexapetalusine E）；化合物3～8为首次从鹰爪花属植物中分离得到，化合物7和8对HepG2细胞表现出明显的细胞毒性。

Objective To investigate the chemical constituents and cytotoxic activities of Artabotrys hexapetalus. Methods The 95% ethanol extracts of the stems and roods of A. hexapetalus were purified on the basis of several chromatographic methods. The structural identification of isolated compounds were based on NMR, HRESIMS and ECD calculation. The cytotoxic activity against three types of tumor cells (HepG2, A549, HCT116) were tested by the CCK-8 methods.Results One undescribed aporphine alkaloid and seven previously reported amide alkaloids were isolated from the 95% ethanol extracts and elucidated as (E)-(6aS,7R)-7-hydroxy-3-methoxy-N-acrylaldehyde-1,2-methylenedioxy oxoaporphine alkaloid (1), trans-N-p-coumaroyltyramine (2), N-trans-3-hydroxy-4-methoxycinnamoyltyramine (3), N-trans-feruloyl-tyramine (4), (E)-3-(4-hydroxy-3-methoxyphenyl)-N-phenethyl-acrylamide (5), northalifoline (6), tribulusamide A (7), and cannabisin F (8). Compounds 7 and 8 showed significantly cytotoxic activity against HepG2 cells with IC₅₀ values of (30.52 ±0.93) μmol/L and (16.26 ±1.97) μmol/L, respectively. Conclusions Compound 1 was an unreported compound named hexapetalusine E, and compounds 3—8 were isolated for the first time from the genus Artabotrys. Compounds 7 and 8 showed significant cytotoxicity against HepG2 cells.

R284.1

国家自然科学基金面上项目（32271523）;2022年银川市学术技术带头人储备工程项目（银人才发[2022]37号）