目的 探讨胶饴对甘草炭纳米类成分（Glycyrrhizae Radix et Rhizoma Carbonisata nano-components，GRRC-NCs）抗胃溃疡的协同增效作用。方法 利用马弗炉高温煅烧法制备甘草炭，加水提取后利用透析法纯化，制备GRRC-NCs溶液；将煮熟大黄米、大麦蘖末混合、糖化、熬煮，制备胶饴。利用扫描电镜（scanning electron microscope，SEM）、紫外-可见分光光谱（ultraviolet-visible spectroscopy，UV-Vis）、荧光光谱（fluorescence spectrum，FL）、X射线光电子能谱（X ray photoelectron spectroscopy，XPS）、傅里叶变换红外光谱（Fourier transform infrared spectroscopy，FTIR）、X射线衍射（X ray diffraction，XRD）技术对胶饴、GRRC-NCs以及不同比例混合物进行表征。构建大鼠胃溃疡模型，通过比较各组大鼠胃溃疡程度、病理学变化、血清中白细胞介素-10（interleukin-10，IL-10）和胃组织中肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、IL-1β、大鼠前列素E₂（prostaglandin E₂，PGE₂）、超氧化物歧化酶（superoxide dismutase，SOD）、丙二醛（malondialdehyde，MDA）、过氧化氢酶（catalase，CAT）、还原型谷胱甘肽（reduced glutathione，GSH）的水平，评价胶饴对GRRC-NCs抗胃溃疡的协同增效作用。结果 通过初步表征推测出，胶饴与GRRC-NCs混合后引起了物质结构的变化，其中胶饴与GRRC-NCs 1∶2混合物的结构状态更为稳定。动物实验结果显示，与胶饴和GRRC-NCs单用相比，胶饴与GRRC-NCs 1∶2混合溶液能有效减轻胃组织损伤，显著降低IL-1β、TNF-α水平（P＜0.05），明显升高IL-10、PGE2水平（P＜0.05）；与模型组比较，胶饴与GRRC-NCs 1∶2混合溶液能显著升高SOD、GSH、CAT水平（P＜0.001），大幅下调MDA水平（P＜0.001）。结论 胶饴对GRRC-NCs抗胃溃疡有协同增效作用，在胶饴与GRRC-NCs 1∶2时抗胃溃疡的药效最好，为小建中汤中胶饴与炙甘草的配伍比例提供了实验依据。

Objective To investigate the synergistic effect of Jiaoyi (胶饴, JY) on Gancaotan (Glycyrrhizae Radix et Rhizoma Carbonisata nano-components, GRRC-NCs) against gastric ulcer. Methods The GRRC-NCs solution is prepared by high temperature calcination in Muffle furnace, extracted with water and purified by dialysis. The cooked rhubarb rice and barley tiller are mixed, saccharated and boiled to prepare Jiaoyi. Scanning electron microscope (SEM), ultraviolet-visible spectroscopy (UV-Vis), fluorescence spectrum (FL), X-ray photoelectron spectroscopy (XPS) , Fourier transform infrared (FTIR), X-ray diffraction (XRD) were used to characterize Jiaoyi, GRRC-NCs and their mixtures in different proportions. The gastric ulcer model of rats was used to compare degree of gastric ulcer, pathological changes, levels of interleukin-10 (IL-10) in the serum, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and prostaglandin E₂ (PGE₂), superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), reduced glutathione (GSH) in gastric tissue to evaluate the synergistic effect of Jiaoyi on GRRC-NCs against gastric ulcer. Results It was inferred from the preliminary characterization that the mixing of Jiaoyi and GRRC-NCs caused material structural changes, and the structural state of the mixture of Jiaoyi and GRRC-NCs 1:2 was more stable. The results of animal experiments showed that compared with Jiaoyi and GRRC-NCs alone, Jiaoyi and GRRC-NCs 1:2 mixed solution could effectively reduce gastric tissue damage, significantly reduce the levels of IL-1β and TNF-ɑ (P< 0.05), and significantly increase the levels of IL-10 and PGE2 (P < 0.05). Compared with the model group, the mixed solution of Jiaoyi and GRRC-NCs 1:2 significantly increased the levels of SOD, GSH and CAT (P < 0.001), and significantly decreased the levels of MDA (P< 0.001).Conclusion Jiaoyi has a synergistic effect on the anti-ulcer effect of GRRC-NCs, and the anti-ulcer effect is the best when Jiaoyi and GRRC-NCs 1:2, which provides a certain experimental basis for revealing the compatibility significance of Jiaoyi and Glycyrrhizae Radixet Rhizoma Praeparatacum Melles in Xiaojianzhong Decoction.

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国家中医药管理局岐黄学者支持项目（90020172120064）;中央高校基本科研业务费专项资金资助项目（2024-JYB-JBZD-040，2024-JYB-JBZD-045）