目的 对不同生长期滨蒿Artemisia scoparia进行质量评价，为确定滨蒿最佳采收期和临床用药选择提供参考。方法 采用超快速液相色谱-三重四级杆飞行时间质谱（Ultra-fast liquid chromatography-quadrupole time-of-flight mass spectrometry，UFLC-Q-TOF-MS/MS），分别在负离子模式和正离子模式下测定，对春季和秋季采集的滨蒿中所含成分进行鉴定并进行成分组成比较分析。采用HPLC-DAD，在2个波长下对5～11月每月采集的滨蒿中的有机酸类成分绿原酸、新绿原酸、隐绿原酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸，黄酮类成分金丝桃苷和芹菜素-6,8-C-二葡萄糖苷及香豆素类成分滨蒿内酯进行含量测定并对含量变化进行动态比较分析。结果 春季和秋季采集的滨蒿中共鉴定化学成分122个，包括有机酸类成分48个，黄酮类成分31个，香豆素类成分5个，木脂素类成分3个，简单苯丙素类成分13，色原酮类成分2个，氨基酸类成分2个，对羟基苯乙酮类成分3个及其他类成分15个，其中15个成分采用对照品比对确认；检测到的122个成分中，103个为春季和秋季采集的滨蒿中的共有成分，3个成分仅在春季采集的滨蒿中检测到，而另外16个成分仅在秋季采集的滨蒿中检测到。不同生长期滨蒿中香豆素类成分滨蒿内酯含量变化最大，5～8月低于定量限，9～11月含量先增后降，10月初含量最高；有机酸类成分春季和夏季含量较高，6月初总含量达到峰值；黄酮类成分含量5～9月逐渐增加，10月初达到峰值后又迅速降低。结论 春季和秋季采集的滨蒿中成分组成存在一定差异；不同生长期滨蒿中所含成分含量差异较大，春季和秋季最佳采收期分别为有机酸类成分含量最高的6月初及香豆素类成分和黄酮类成分含量最高的10月初。

Objective To evaluate the quality of Artemisia scopariaat different growth periods, and to provide reference for determining the optimal harvesting period of A. scoparia and clinical medication selection. Methods Using ultra-fast liquid chromatography-quadrupole time-of-flight mass spectrometry (UFLC-Q-TOF-MS/MS) in both negative and positive ion modes, the components in A. scoparia collected in spring and autumn were identified and compared for compositional analysis. Using HPLC-DAD, the contents of organic acids named as chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, and 4,5-dicaffeoyl quinic acid, flavonoids named as hyperoside and apigenin 6,8-di-C-β-D-glucoside, and coumarins named as scoparone in A. scoparia collected monthly from May to November were determined at two wavelengths, and dynamic comparative analysis was conducted on the content changes.Results A total of 122 chemical components were identified in A. scoparia collected in spring and autumn, including 48 organic acids, 31 flavonoids, five coumarins, three lignans, 13 simple phenylpropanoids, two chromones, two amino acids, three p-hydroxyacetophenones and 15 others, with 15 components were confirmed through comparison with reference substances. Among the 122 detected components, 103 were common components in A. scoparia collected in spring and autumn, three components were only detected in A. scoparia collected in spring, while the other 16 components were only detected in A. scoparia collected in autumn. The content of a coumarins named scoparone in A. scoparia varied the most during different growth periods, with its content below the LOQ from May to August, increasing first and then decreasing from September to November, and reaching its highest level in early October. The content of organic acids is relatively high in spring and summer, and the total content reaches its peak in early June. The content of flavonoids gradually increases from May to September, and reaches its peak in early October before rapidly decreasing.Conclusion There are certain differences in the composition of components in A. scoparia collected in spring and autumn. There are significant differences in the content of components in A. scoparia during different growth periods. The best harvesting periods for A. scoparia in spring and autumn are early June, which has the highest content of organic acids, and early October, which has the highest content of coumarins and flavonoids, respectively.

R286.2

江苏省重点研发计划（社会发展）项目（BE2018674）