目的 找到适宜桃儿七Sinopodophyllum hexandrum幼苗成活和植株生长的育苗基质和种植施肥量，为高效种植和植株生长提供技术支撑。方法 以桃儿七成熟种子和温室植株为材料，分别利用6种育苗基质（S1～S6）和施用4个水平施肥量（F1～F4），测定幼苗成活特性、植株生长特性及主要活性物质含量。结果 不同育苗基质和施肥量对桃儿七幼苗成活和生长及主要活性物质积累具有显著影响。S2育苗基质优于其他处理，幼苗成活率相对于对照增加22.90倍。F1和F2施肥量（2 400和4 200 kg/hm²）优于其他处理，根干质量相对于对照分别增加1.12和1.34倍；F1施肥量较有利于主要活性物质积累，以单株计算，根中鬼臼毒素、4'-去甲基表鬼臼毒素、异槲皮苷、槲皮素、总黄酮类和总酚类化合物含量相对于对照分别增加1.08、1.68、1.28、1.39、1.42和1.47倍。结论 适宜的育苗基质和施肥量可显著促进桃儿七幼苗成活、植株生长及主要活性物质的积累。为后续桃儿七生态种植提供科学依据和参考。

Objective To find out suitable seedling substrates and planting fertilizer amounts for seedling survival and plant growth and provide technical supports for efficient planting and production of Taoerqi (Sinopodophyllum hexandrum). Methods A total of six different seedling substrates (S1 to S6) and four different fertilizer amounts (F1 to F4) were applied in the mature seeds and greenhouse plants respectively, and germination characteristics, plant growth and main bioactive metabolite contents in S. hexandrum were examined. Results There were significant effects of seedling substrates and fertilizer amounts on seed germination, plant growth and main bioactive metabolites accumulation in S. hexandrum. The S2 treatment was more suitable for seedling survival than other treatments, with a 22.90-fold increase compared with the control (CK). The F1 and F2 (2 400 and 4 200 kg/hm²) were more suitable for plant growth than other treatments, with a 1.12- and 1.34-fold increase of dry weight of rhizomes compared with the CK. The F1 treatment was suitable for the main bioactive metabolite accumulation, with a 1.08-, 1.68-, 1.28-, 1.39-, 1.42- and 1.47-fold increase of podophyllotoxin, 4'-demethylepipodophyllotoxin, isoquercetin, quercetin, total flavonoids and total phenolics in roots on a per plant basis compared with the CK, respectively. Conclusion The seedling survival, plant growth and bioactive metabolite accumulation could be significantly improved by suitable seedling substrates and fertilizer amounts, these findings will provide scientific references for ecological planting of S. hexandrum in the future.

R286.2

国家中药材产业技术体系（CARS-21）;道地药材生态种植及质量保障项目（202203002）