目的 建立灵香草Lysimachia foenum-graecum一测多评法（quantitative analysis of multi-components by single marker，QAMS）多指标成分定量控制方法，采用多元统计分析模型评价不同产地灵香草质量差异。方法 以Prep Scalar C18柱为色谱柱，乙腈-0.2%磷酸为流动相（梯度洗脱），以槲皮素和熊果酸为内参物，采用QAMS法同时测定灵香草中绿原酸、杨梅素、槲皮素、山柰酚、foenumoside B、零陵香皂苷C、齐墩果酸、熊果酸、豆甾醇和β-谷甾醇含量，同时用外标法验证QAMS法的准确性。依据《中国药典》检测浸出物、总灰分和酸不溶性灰分。进一步应用主成分分析（principal component analysis，PCA）、因子分析（factor analysis，FA）和正交偏最小二乘法判别分析（orthogonal partial least squares discriminant analysis，OPLS-DA）法对15批不同产地的灵香草进行区分比较，寻找差异因子。结果 建立的相对校正因子可用于定量分析，且耐用性良好；QAMS法与外标法结果无显著差异，但各批次间差异较大。PCA结果显示15批灵香草聚为3类，FA结果显示广东和广西产地的灵香草质量较优，OPLS-DA结果显示foenumoside B、山柰酚、零陵香皂苷C、绿原酸、槲皮素和豆甾醇是影响灵香草产品质量的差异性标志物。结论 基于多指标成分定量、多元统计分析评价灵香草质量的方法，为灵香草质量标准统一和提升提供基础资料。

Objective To establish a quantitative control method for multi-index components of Lysimachia foenum-graecum by QAMS, and to evaluate the quality difference of Lysimachia foenum-graecum from different producing areas by multivariate statistical analysis model. Method A Prep Scalar C₁₈ column was used as the chromatographic column, and acetonitrile-0.2% phosphoric acid was used as the mobile phase (gradient elution). The contents of chlorogenic acid, myricetin, quercetin, kaempferol, foenumoside B, lysimachigenoside C, oleanolic acid, ursolic acid, stigmasterol and β-sitosterol in Lysimachia foenum-graecum were simultaneously determined by QAMS method with quercetin and ursolic acid as internal reference. The accuracy of QAMS method was verified by external standard method. Meanwhile, extract, total ash and acid insoluble ash were detected according to Chinese Pharmacopoeia. The PCA, FA and OPLS-DA were used to distinguish and compare 15 batches of Lysimachia foenum-graecum from different producing areas, and explore the difference factors for quality evaluation of Lysimachia foenum-graecum. Results The established relative correction factor can be used for quantitative analysis and has good durability. The results of QAMS method were close to those of external standard method, but the difference between batches was large. The PCA results showed that 15 batches of Lysimachia foenum-graecum were clustered into three categories. The FA results showed that the quality of Lysimachia foenum-graecum from Guangdong and Guangxi was better. The results of OPLS-DA showed that foenumoside B, kaempferol, lysimachigenoside C, chlorogenic acid, quercetin and stigmasterol were differential markers affecting the quality of Lysimachia foenum-graecum. Conclusion The method of evaluating the quality of Lysimachia foenum-graecum based on HPLC, multivariate statistical analysis provides basic data for the screening of Lysimachia foenum-graecum and the unification and improvement of quality standards.

R286.2

河南省高等学校重点科研指导计划项目(20B550007)