目的 研究顶花板凳果Pachysandra terminalis的化学成分。方法 采用硅胶和凝胶柱色谱以及半制备液相色谱等方法进行分离纯化，并结合其理化性质及NMR、IR、MS等谱学技术进行结构解析。采用CCK-8法测试单体化合物体外抗人肺癌H460细胞活性。结果 从顶花板凳果80%乙醇水提取物的二氯甲烷部位分离得到6个化合物，分别鉴定为顶花板凳果酮A（1）、顶花板凳果醇A（2）、(E)-4-(2,2,6-三甲基环己基)丁-3-烯-2-酮（3）、(3S,5R,6S,9R)-3,6-二羟基-5,6-二氢-β-香堇酮（4）、(＋)-博氏木林素（5）、蚱蜢酮（6）。化合物5抑制H460细胞的半数抑制浓度（median inhibition concentration，IC₅₀）值为（12.58±1.37）μmol/L。结论 化合物1和2为新化合物，分别命名为顶花板凳果酮A（pactermone A）和顶花板凳果醇A（pactermol A），化合物3为新天然产物，化合物4～6为首次从板凳果属植物中分离得到。化合物5对H460细胞具有一定的抑制作用。

Objective To study the chemical composition of Pachysandrae terminalis. Methods The separation and purification were conducted by silica gel column chromatography, gel column chromatography and semi-preparative liquid chromatography. The structures were analyzed by physicochemical properties and spectroscopic techniques such as NMR, IR and MS. The isolated compounds were tested for their cytotoxic activities in H460 tumor cells by CCK-8 method. Results A total of six compounds were isolated from the dichloromethane extract of P. terminalis and identified as pactermone A (1), pactermol A (2), (E)-4-(2,2,6-trimethylcyclohexyl)but-3-en-2-one (3), (3S,5R,6S,9R)-3,6-dihydroxy-5,6-dihydro-β-ionone (4), (+)-boscialin (5), grasshopper ketone (6). Compound 5 showed inhibitory effects in H460 cells with IC₅₀ value of (12.58 ±1.37) μmol/L. Conclusion Compounds 1 and 2 were identified as two new compounds, named pactermone A and pactermol A, respectively. Compound 3 was a new natural product. Compounds 4—6 were isolated from genus Pachysandra for the first time. Compound 5 showed inhibitory effects on H460 cells.

R284.1

国家自然科学基金项目(82104368);陕西中医药大学创新团队项目(2023-CXTD-05)