目的 研究鉴定巴戟天Morinda officinalis AP2/ERF转录因子家族，为巴戟天次生代谢调控网络提供理论基础。方法 通过对巴戟天AP2/ERF转录因子家族的探索，利用TBtools、MEGA等生物信息分析软件对巴戟天基因组基因结构注释信息等文件进行基本分析、进化分析，得出巴戟天中AP2/ERF家族的基因，以及这些基因中内含子排列情况、共线性分析的结果，并且通过进化树的构建可视化AP2/ERF基因家族的分类。结果 初步筛选出可能调节巴戟天生长发育和代谢的转录因子。在巴戟天基因组中，鉴定到有131条基因属于AP2/ERF基因家族成员，通过基因结构注释文件，得到MoAP2/ERF在染色体定位分析、系统发育进化树、内含子外显子模式、motif、保守结构域分析和共线性分析等，最终根据MoAP2/ERF的表达量确认13条在巴戟天根部高表达的转录因子。结论 通过生物信息分析筛选出在根部特异性表达的基因13条巴戟天AP2/ERF转录因子，为增加巴戟天主要有效成分产率奠定理论基础，为后续研究提供信息。

Objective To identify the Bajitian (Morinda officinalis) AP2/ERF transcription factor family for providing a theoretical basis for M. officinalis secondary metabolic regulatory network. Methods In this study, the genes of the AP2/ERF family in M. officinalis, as well as the intron arrangement and collinearity analysis results of these genes, were obtained by exploring the AP2/ERF transcription factor family in M. officinalis, and the classification of AP2/ERF gene family was visualized through the construction of phylogenetic tree. Results Transcription factors that may regulate the growth, development and metabolism of M. officinalis were preliminarily screened. In the genome of M. officinalis, 131 genes belonging to the AP2/ERF gene family were identified, and chromosome localization analysis, phylogenetic evolution tree, intron exon pattern, motif, conserve domain analysis and collinearity analysis of MoAP2/ERF were obtained through gene structure annotation files, and finally 13 transcription factors with high expression in the root of M. officinalis were confirmed. Conclusion A total of 13 AP2/ERF transcription factors of M. officinalis were screened out by bioinformatics analysis, which laid a theoretical foundation for increasing the yield of the main active components of M. officinalis and provided information for follow-up research.

R286.12

科技部基础资源调查重点专项(2018FY100700);广东省基础与应用基础研究基金项目(2022A1515010646);广东省中医药管理局面上项目(20241085);广东省级大学生创新训练项目(202310572062)