目的 采用指纹图谱、多指标成分定量与化学计量法相结合的方法，评价不同产地延胡索Corydalis yanhusuo的质量，为其进一步开发利用提供依据。方法 采用HPLC法，流动相为乙腈-0.1 mol/L磷酸水溶液进行梯度洗脱，柱温30 ℃，体积流量0.8 mL/min，检测波长280 nm，对14批不同产地延胡索药材建立指纹图谱及原阿片碱、延胡索乙素、盐酸药根碱、盐酸巴马汀、脱氢紫堇碱5种有效成分含量测定，运用聚类分析（hierarchical cluster analysis，HCA）、主成分分析（principal component analysis，PCA）和正交偏最小二乘法-判别分析（orthogonal partial least squares-discriminant analysis，OPLS-DA）对延胡索进行化学计量分析。结果 建立了14批延胡索药材的指纹图谱，相似度为0.739～0.991，共标定16个共有峰，指认了5个成分，含量测定结果表明不同产地延胡索中化学成分含量存在明显差异；HCA分析14批延胡索明显分为2类；PCA得到4个主成分的累积方差贡献率为86.751%；OPLS-DA表明延胡索乙素和脱氢紫堇碱可能是影响延胡索药材质量的差异标志物。结论 建立的延胡索HPLC指纹图谱及5个成分含量测定方法的专属性强，且准确、可靠，结合化学计量法可用于延胡索的药材鉴别和质量控制。

Objective Based on the method of HPLC fingerprint, multi-index component quantification and chemometric analysis, the quality of Yanhusuo (Corydalis Rhizoma) from different habitats was evaluated to provide a basis for its further development and utilization. Methods HPLC method was used with acetonitrile-0.1 mol/L phosphoric acid water solution as the mobile phase for gradient elution. The column temperature was 30 ℃, the flow rate was 0.8 mL/min, and the detection wavelength was 280 nm. The fingerprints were established for 14 batches of Rhizoma corydalis from different habitats and contents of five effective components of protopine, tetrahydropalmatine, jatrorrhizine hydrochloride, palmatine hydrochloride and dehydrocorydaline were determined. The hierarchical cluster analysis (HCA), principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were used to analyze the stoichiometry of Corydalis Rhizoma. Results The fingerprints of 14 batches of Corydalis Rhizoma were established, with similarity ranging from 0.739 to 0.991. A total of 16 common peaks were identified, and five components were identified. The results of content determination showed that there were significant differences in the content of chemical components in Corydalis Rhizoma from different habitats. HCA analysis showed that 14 batches of Corydalis Rhizoma were clearly divided into two categories. PCA obtained four principal components with a cumulative contribution rate of 86.751%. OPLS-DA indicated that tetrahydropalmatine and dehydrocorydaline may be the markers of quality difference of Corydalis Rhizoma. Conclusion The established HPLC fingerprint and content determination method of five components of Corydalis Rhizoma had strong specificity, accuracy, and reliability, and can be used for identification and quality control of Corydalis Rhizoma by combining chemometric analysis.

R286.2

四川省博士后科学基金资助项目(TB2023050);四川省中医药管理局专项课题(2023MS084;2023ZD02);国家级大学生创新创业计划项目(202413705031);“成医英才”登峰计划人才项目(2024qnGzn14)