目的 通过对金银花Lonicerae Japonicae Flos-蒲公英Taraxaci Herba提取液的相态拆分，研究提取液及不同相态（真溶液相态、纳米相态、沉淀相态）中存在的主要成分（绿原酸、菊苣酸、异绿原酸A、异绿原酸C）含量及其对痤疮丙酸杆菌的抑制作用，筛选金银花-蒲公英提取液治疗痤疮的“有效相态”。方法 采用透析-离心方法对金银花-蒲公英提取液进行相态拆分，对其外观形态、粒径、多分散指数（polydispersity index，PDI）、ζ电位进行表征，并通过HPLC法对各相态主要成分含量进行测定以及各相态对痤疮丙酸杆菌最小抑菌浓度（minimum inhibitory concentration，MIC）及最小杀菌浓度（minimum bactericidal concentration，MBC）进行测定。结果 金银花-蒲公英提取液各相态的粒径大小为总提取液＞沉淀相态＞纳米相态，真溶液相态未测定到粒径；各相态的PDI大小为纳米相态＞总提取液＞沉淀相态；各相态ζ电位大小为沉淀相态＞总提取液＞纳米相态＞真溶液相态；各相态绿原酸质量分数排序为纳米相态＞真溶液相态＞沉淀相态；菊苣酸质量分数排序为纳米相态＞真溶液相态＞沉淀相态；异绿原酸A质量分数排序为纳米相态＞真溶液相态＞沉淀相态；异绿原酸C质量分数排序为纳米相态＞沉淀相态＞真溶液相态；金银花-蒲公英总提取液与纳米相态抑菌结果一致，效果最好；杀菌效果为总提取液最佳，其次为纳米相态。结论 物理表征结果显示，金银花-蒲公英提取液中纳米相态体系稳定性较差，可能发生了团聚现象，但其主要成分质量分数最高，抑制痤疮丙酸杆菌效果最佳，能够证实纳米相态为金银花-蒲公英提取液治疗痤疮的有效相态，为中药提取工艺的科学性和合理性提供基础。

Objective Through the phase states splitting of Jinyinhua (Lonicerae Japonicae Flos, LJF)-Pugongying (Taraxaci Herba, TH) extract, the content of the main components (chlorogenic acid, chicoric acid, isochlorogenic acid A, isochlorogenic acid C) in the extract and different phase states (true solution phase, nano phase, precipitated phase) and their inhibitory effect on Propionibacterium acnes were studied, and the “effective phase state” of LJF-TH extract in the treatment of acne was screened. Methods The phase states separation of LJF-TH extract was carried out by dialysis-centrifugation method, and its appearance, particle size, polydispersity index (PDI), and ζ potential were characterized, and the contents of main components in each phase states were determined by HPLC, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of P. acnes in each phase states were determined. Results The particle size of each phase states of LJF-TH extract was the total extract > precipitated phase > nano phase, and the particle size of the true solution phase was not measured. The size of the PDI of each phase states was nano phase > total extract > precipitated phase. The ζ potential magnitude of each phase states was precipitated phase > total extract > nano phase > true solution phase. The content of chlorogenic acid in each phase states was ranked as nano phase > true solution phase > precipitated phase. The order of cichoric acid content was nano phase > true solution phase > precipitated phase. The content of isochlorogenic acid A was ranked as nano phase > true solution phase > precipitated phase. The content of isolochlorogenic acid C was ranked as nano phase > precipitated phase > true solution phase. The antibacterial results of the LJF-TH total extract were consistent with those of the nano phase, and the effect was the best. The sterilization effect was the best for the total extract, followed by the nano phase. Conclusion The physical characterization results showed that the nano phase system in LJF-TH extract was less stable and may have agglomerated phenomenon, but its main component content was the highest, and the inhibition effect of P. acnes was the best, which could confirm that the nano phase was an effective phase in the treatment of acne in LJF-TH extract, providing a basis for the science and rationality of extraction technology of traditional Chinese medicine.

R283.6

江西中医药大学博士启动基金（2023WBZR-007）;2023年度中药学重点学科项目（2023jzzdxk016）;国家中医药管理局高水平中医药重点学科建设项目（国中医药人教函［2023］85号）;江西中医药大学校级研究生创新专项（JZYC22S74）;2023年全国中药特色技术人才培训项目（T20234832005）